Literature summary for 2.3.1.43 extracted from

Gunawardane, R.; Fordstrom, P.; Piper, D.; Masterman, S.; Siu, S.; Liu, D.; Brown, M.; Lu, M.; Tang, J.; Zhang, R.; Cheng, J.; Gates, A.; Meininger, D.; Chan, J.; Carlson, T.; Walker, N.; Schwarz, M.; Delaney, J.; Zhou, M.
Agonistic human antibodies binding to lecithin-cholesterol acyltransferase modulate high density lipoprotein metabolism (2016), J. Biol. Chem., 291, 2799-2811 .

Search for more literature for 2.3.1.43

Activating Compound

Activating Compound	Comment	Organism	Structure
antibody 27C3	antibody 27C3 binds to and substantially enhances the activity of the enzyme	Macaca fascicularis
antibody 27C3	antibody 27C3 binds to and substantially enhances the activity of the enzyme. 24 h after dosing 27C3, a 2.3fold increase of the serum enzyme enzyme activity is observed, followed by a steady elevation of enzyme activity that is sustained for about 20 days before gradually returning to baseline levels	Homo sapiens
additional information	antibody 14F11 does not enhances the enzyme activity	Homo sapiens
additional information	antibody 14F11 does not enhances the enzyme activity	Macaca fascicularis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HEK-293S cells	Homo sapiens
expressed in HEK-293S cells	Macaca fascicularis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with antibody 27C3, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.0) and 5% (w/v) PEG 20000	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C31Y	the mutant has an about 10fold higher cholesterol esterification activity compared with wild type	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
antibody 25B7	-	Homo sapiens
antibody 25B7	-	Macaca fascicularis
additional information	antibody 14F11 does not inhibit the enzyme activity	Homo sapiens
additional information	antibody 14F11 does not inhibit the enzyme activity	Macaca fascicularis

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P04180	-	-
Macaca fascicularis	A0A2K5WDQ7	-	-

Purification (Commentary)

Purification (Comment)	Organism
protein A affinity column chromatography, HisTrap column chromatography and Superdex S200 gel filtration	Homo sapiens
protein A affinity column chromatography, HisTrap column chromatography and Superdex S200 gel filtration	Macaca fascicularis

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood plasma	-	Homo sapiens	-
blood plasma	-	Macaca fascicularis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alpha-phosphatidylcholine type XVI-E + cholesterol	-	Homo sapiens	1-acylglycerophosphocholine + cholesteryl ester	-	?
L-alpha-phosphatidylcholine type XVI-E + cholesterol	-	Macaca fascicularis	1-acylglycerophosphocholine + cholesteryl ester	-	?

Synonyms

Synonyms	Comment	Organism
LCAT	-	Homo sapiens
LCAT	-	Macaca fascicularis
lecithin-cholesterol acyltransferase	-	Homo sapiens
lecithin-cholesterol acyltransferase	-	Macaca fascicularis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)