Application | Comment | Organism |
---|---|---|
drug development | a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis | Homo sapiens |
medicine | a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene lcat, use of baculovirus as a transducing vector to express the C-or N-terminally His-tagged enzyme in insect cell line Sf9, and to transiently express tagged LCAT in human HEK-293 GnTI cells, deficient in N-acetylglucosaminyl transferase I and expression of the recombinant protein as a high-mannose glycoform (of the MAN9GlcNAc2 or MAN5GlcNAc2 types) suitable for deglycosylation by Endo H, which leaves a single N-acetyl glucosamine (GlcNAc) residue at the glycosylation sites, to avoid protein aggregation coccuring with fully deglycosylated protein. Or expression in HEK-293 6E cells. TEV or Factor Xa sites are introduced for tag cleavage. The enzyme is secreted. Method development and evaluation, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C31Y | site-directed mutagenesis, the mutation renders the enzyme more stable and active than the native form | Homo sapiens |
additional information | removal of a proline rich region (residues 402 to 416) on the C-terminus | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | 62000 | recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + a sterol | Homo sapiens | - |
1-acylglycerophosphocholine + a sterol ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04180 | gene lcat | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is highly glycosylated with four potential N-linked glycosylation sites and two putative O-linked sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant partially deglycosylated His-tagged enzyme from HEK-293 GnTI cells by nickel affinity chromatography to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
liver | produced predominantly in the liver and secreted as a circulating protein into the serum | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + a sterol | - |
Homo sapiens | 1-acylglycerophosphocholine + a sterol ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LCAT | - |
Homo sapiens |
lecithin-cholesterol acyltransferase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | LCAT is a member of the alpha/beta hydrolase family | Homo sapiens |
physiological function | a key enzyme in the esterification of cholesterol and its subsequent incorporation into the core of high density lipoprotein (HDL) particles. The enzyme is also also involved in reverse cholesterol transport, the mechanism by which cholesterol is removed from peripheral cells and transported to the liver for excretion | Homo sapiens |