Activating Compound | Comment | Organism | Structure |
---|---|---|---|
apolipoprotein A-I | - |
Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | the recombinant enzyme is utilized for enzyme replacement therapy in case of congenital enzyme deficiency | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
optimization of expression in human lung cell line H1299, enzyme is secreted to the culture medium | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00056 | - |
1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine | fluorescent assay, pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
0.0006 | - |
phosphatidylcholine | radioassay, pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
64000 | - |
x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure | ? | - |
? | |
phosphatidylcholine + cholesterol | Homo sapiens | - |
1-acylglycerophosphocholine + cholesteryl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04180 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | analysis of sugar composition, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from human lung cell line H1299 culture medium | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities produced by different lung cell clones expressing the enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-bis[4-(1-pyreno)butanoyl]-sn-glycero-3-phosphocholine + cholesterol | fluorescent substrate assay | Homo sapiens | ? | - |
? | |
additional information | enzyme deficiency can cause corneal opacity, proteinuria, anemia, and kidney failure | Homo sapiens | ? | - |
? | |
phosphatidylcholine + cholesterol | - |
Homo sapiens | 1-acylglycerophosphocholine + cholesteryl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, recombinant glycosylated enzyme, SDS-PAGE, x * 44000, recombinant deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LCAT | - |
Homo sapiens |
lecithin:cholesterol acyltransferase | - |
Homo sapiens |