Activating Compound | Comment | Organism | Structure |
---|---|---|---|
apolipoprotein A-I | absolute requirement with phosphatidylcholine-cholesterol vesicles as a substrate | Homo sapiens | |
additional information | apo-A-II, apo-C-III and apo-D inhibit activation of enzyme by apo-A-1 | Homo sapiens |
General Stability | Organism |
---|---|
inactivation at the air-water interface is prevented by buffered media of low ionic strength | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Homo sapiens | |
carnitine | - |
Homo sapiens | |
chlorpromazine | - |
Homo sapiens | |
cysteine | - |
Homo sapiens | |
diisopropyl fluorophosphate | - |
Homo sapiens | |
p-hydroxymercuribenzoate | - |
Homo sapiens | |
reduced glutathione | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Homo sapiens |
67000 | - |
size exclusion chromatography | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + sterol | Homo sapiens | enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure | 1-acylglycerophosphocholine + sterol ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 25% carbohydrate | Homo sapiens |
glycoprotein | with a 5% sialic acid content | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
at 37°C and pH: 7.4 | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 0.4 mM phosphate buffer, 4 mM 2-mercaptoethanol, under N2, 4 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lecithin-cholesterol single bilayer vesicles as substrate | Homo sapiens | ? | - |
? | |
phosphatidylcholine + sterol | cholesterol as substrate | Homo sapiens | 1-acylglycerophosphocholine + sterol ester | - |
r | |
phosphatidylcholine + sterol | enzyme is believed to act on the surface of high-density lipoproteins, catalyzes the hydrolysis of fatty acids of the sn-2-position of phosphatidylcholine and transfers the fatty acid to free cholesterol to form cholesteryl ester, transesterification is the major source of plasma cholesteryl ester in human, enzyme has the key role in the transport of cholesterol from peripheral tissues to the liver, in the interconversion of HDL subclasses and the maintenance of lipoprotein structure | Homo sapiens | 1-acylglycerophosphocholine + sterol ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
transesterification of high-density lipoproteins and liposomes | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
apolipoprotein A1 | - |
Homo sapiens |