Cloned (Comment) | Organism |
---|---|
gene DAT, DNA amd amino acid sequence determination and analysis, DAT functionally complements the lethality resulting from the loss of both dihydroxyacetone phosphate and glycerol-3-phosphate acyltransferase activities in yeast, recombinant DAT exhibits biochemical properties similar to those of the native enzyme of the promastigote stage parasites | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a DELTAmdat/DELTAlmdat null mutant results in complete abrogation of the parasite dihydroxyacetone phosphate acyltransferase activity, lack of LmDAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice, phenotype, overview | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Leishmania major | |
0.69 | - |
dihydroxyacetone phosphate | pH 7.5, 30°C, recombinant enzyme | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Leishmania major | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + acyl-CoA | Leishmania major | the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice | 1-acyl-glycerone phosphate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
gene DAT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | promastigote stage parasites | Leishmania major | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + acyl-CoA | the enzyme performs the initial and obligatory step in the biosynthesis of ether lipids in most organisms that synthesize alkylglycerolipids and is important for rapid growth and survival at high cell density and essential for virulence, overview, lack of DAT causes a major alteration in parasite division during the logarithmic phase of growth, an accelerated cell death during stationary phase, and loss of virulence in mice | Leishmania major | 1-acyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + arachidoyl-CoA | - |
Leishmania major | 1-arachidoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + lauroyl-CoA | - |
Leishmania major | 1-lauroyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + linoleoyl-CoA | - |
Leishmania major | 1-linoleoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + myristoyl-CoA | - |
Leishmania major | 1-myristoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + oleoyl-CoA | - |
Leishmania major | 1-oleoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + palmitoleoyl-CoA | - |
Leishmania major | 1-palmitoleoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + palmitoyl-CoA | palmitoyl-CoA is the preferred acyl-CoA substrate | Leishmania major | palmitoyl-glycerone phosphate + CoA | - |
? | |
dihydroxyacetone phosphate + stearoyl-CoA | - |
Leishmania major | 1-stearoyl-glycerone phosphate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHAP acyltransferase | - |
Leishmania major |
DHAPAT | - |
Leishmania major |
dihydroxyacetone phosphate acyltransferase | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania major |