Literature summary for 2.3.1.42 extracted from

Liu, D.; Nagan, N.; Just, W.W.; Rodemer, C.; Thai, T.P.; Zoeller, R.A.
Role of dihydroxyacetonephosphate acyltransferase in the biosynthesis of plasmalogens and nonether glycerolipids (2005), J. Lipid Res., 46, 727-735.

Search for more literature for 2.3.1.42

Cloned(Commentary)

Cloned (Comment)	Organism
expression in CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or dcreased in both cases	Homo sapiens
expression of the human enzyme encoding cDNA in a CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or decreased in both cases	Cricetulus griseus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Cricetulus griseus	5777	-
peroxisome	-	Homo sapiens	5777	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + glycerone phosphate	Cricetulus griseus	enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis	CoA + acylglycerone phosphate	-	?
acyl-CoA + glycerone phosphate	Homo sapiens	enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis	CoA + acylglycerone phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	-	-
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	CHO-K1 cell line	Cricetulus griseus	-
CHO-K1 cell	-	Cricetulus griseus	-
ovary	-	Cricetulus griseus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00004	0.00008	CHO-NRe1-4 cell expressing the human enzyme	Cricetulus griseus
0.00004	0.00008	CHO-NRe1-4 cell expressing the human enzyme	Homo sapiens
0.00042	-	CHO-K1 wild-type cell	Cricetulus griseus
0.00245	-	CHO-NRe1-4 cell overexpressing the human enzyme	Cricetulus griseus
0.00245	-	CHO-NRe1-4 cell overexpressing the human enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + glycerone phosphate	-	Cricetulus griseus	CoA + acylglycerone phosphate	-	?
acyl-CoA + glycerone phosphate	-	Homo sapiens	CoA + acylglycerone phosphate	-	?
acyl-CoA + glycerone phosphate	enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis	Cricetulus griseus	CoA + acylglycerone phosphate	-	?
acyl-CoA + glycerone phosphate	enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis	Homo sapiens	CoA + acylglycerone phosphate	-	?

Synonyms

Synonyms	Comment	Organism
DHAPAT	-	Cricetulus griseus
DHAPAT	-	Homo sapiens
dihydroxyacetonephosphate acyltransferase	-	Cricetulus griseus
dihydroxyacetonephosphate acyltransferase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Cricetulus griseus
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Cricetulus griseus
5.5	-	-	Homo sapiens

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Release 2023.1 (January 2023)