Literature summary for 2.3.1.41 extracted from

Patterson, E.I.; Nanson, J.D.; Abendroth, J.; Bryan, C.; Sankaran, B.; Myler, P.J.; Forwood, J.K.
Structural characterization of beta-ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites (2020), Proteins, 88, 47-56 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta BL21 (DE3)R3 cells	Brucella melitensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with platencin, 7-hydroxycoumarin, and (5-thiophen-2-ylisoxazol-3-yl)methanol, using 20% (w/v) PEG 3350, 100 mM bis-Tris propane pH 8.5, 200 mM sodium malonate	Brucella melitensis

Inhibitors

Inhibitors	Comment	Organism	Structure
(5-thiophen-2-ylisoxazol-3-yl)methanol	-	Brucella melitensis
7-hydroxycoumarin	-	Brucella melitensis
cerulenin	-	Brucella melitensis
platencin	-	Brucella melitensis
platensimycin	-	Brucella melitensis
thiolactomycin	-	Brucella melitensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Brucella melitensis	-	a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Brucella melitensis 2308	-	a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Brucella melitensis	Q2YQQ9	-	-
Brucella melitensis 2308	Q2YQQ9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity column chromatography and gel filtration	Brucella melitensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Brucella melitensis	a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Brucella melitensis 2308	a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Brucella melitensis

Synonyms

Synonyms	Comment	Organism
beta-ketoacyl ACP synthase I	-	Brucella melitensis
FabB	-	Brucella melitensis
KAS I	-	Brucella melitensis

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Release 2023.1 (January 2023)