Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a therapeutic target of designing antibiotics | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Escherichia coli |
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cerulenin | forms two hydrogen bonding interactions with H333 and backbone amide hydrogen of G391 and a hydrophobic interaction with M197, F229, and F231 | Escherichia coli | |
additional information | inhibitor screening of 250000 synthetic compounds, enzyme-inhibitor binding study by NMR spectroscopy, three-dimensional structure of ecKAS I with inhibitors, overview. No or poor inhibition by 2-(2-methylanilino)-N'-[(E)-(6-methylpyridin-2-yl)methylidene]propanehydrazide, (5Z)-5-([4-[(propan-2-yl)oxy]phenyl]methylidene)-3-(prop-2-en-1-yl)-1,3-thiazolidine-2,4-dione, 1-[2-[4-(2-ethoxyethyl)piperazin-1-yl]-2-oxoethyl]pyrrolidin-2-one, 2-(hexylcarbamoyl)cyclohexane-1-carboxylic acid, 2-[(4-methylbenzene-1-sulfonyl)methyl]prop-2-enamide, 2-([(E)-[(3-nitrophenyl)methylidene]amino]oxy)propanoic acid, 2-[4-(furan-2-yl)phenoxy]-2-methylpropanoic acid, and N-(3-pyridinyl) hexanamide, the latter is though active against Staphylococcus aureus and Enterococcus faecalis | Escherichia coli | |
N-(3-pyridinyl)-hexanamide | result of an in silicio screening, antimicrobial activity, binding to beta-ketoacyl-[acyl-carrier-protein] synthase I characterized by saturation-transfer difference NMR spectroscopy | Escherichia coli | |
thiolactomycin | forms a hydrogen bonding with H333 and a hydrophobic interaction of Phe229 and Thr300 with ecKAS I | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | E2QPI4 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Escherichia coli |
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl acyl carrier protein synthase I | - |
Escherichia coli |
beta-ketoacyl-[acyl-carrier-protein] synthase I | - |
Escherichia coli |
KAS I | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | beta-ketoacyl acyl carrier protein synthase I is involved in the elongation of unsaturated fatty acids in bacterial fatty acid synthesis | Escherichia coli |