Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.41 extracted from

  • Lee, J.; Jeong, K.; Lee, J.; Kang, D.; Kim, Y.
    In silico screening of a novel inhibitor of beta-ketoacyl acyl carrier protein synthase i (2011), Bull. Korean Chem. Soc., 32, 1645-1649.
No PubMed abstract available

Application

Application Comment Organism
drug development the enzyme is a therapeutic target of designing antibiotics Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21(DE3) Escherichia coli
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
cerulenin forms two hydrogen bonding interactions with H333 and backbone amide hydrogen of G391 and a hydrophobic interaction with M197, F229, and F231 Escherichia coli
additional information inhibitor screening of 250000 synthetic compounds, enzyme-inhibitor binding study by NMR spectroscopy, three-dimensional structure of ecKAS I with inhibitors, overview. No or poor inhibition by 2-(2-methylanilino)-N'-[(E)-(6-methylpyridin-2-yl)methylidene]propanehydrazide, (5Z)-5-([4-[(propan-2-yl)oxy]phenyl]methylidene)-3-(prop-2-en-1-yl)-1,3-thiazolidine-2,4-dione, 1-[2-[4-(2-ethoxyethyl)piperazin-1-yl]-2-oxoethyl]pyrrolidin-2-one, 2-(hexylcarbamoyl)cyclohexane-1-carboxylic acid, 2-[(4-methylbenzene-1-sulfonyl)methyl]prop-2-enamide, 2-([(E)-[(3-nitrophenyl)methylidene]amino]oxy)propanoic acid, 2-[4-(furan-2-yl)phenoxy]-2-methylpropanoic acid, and N-(3-pyridinyl) hexanamide, the latter is though active against Staphylococcus aureus and Enterococcus faecalis Escherichia coli
N-(3-pyridinyl)-hexanamide result of an in silicio screening, antimicrobial activity, binding to beta-ketoacyl-[acyl-carrier-protein] synthase I characterized by saturation-transfer difference NMR spectroscopy Escherichia coli
thiolactomycin forms a hydrogen bonding with H333 and a hydrophobic interaction of Phe229 and Thr300 with ecKAS I Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli E2QPI4
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Escherichia coli
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Synonyms

Synonyms Comment Organism
beta-ketoacyl acyl carrier protein synthase I
-
Escherichia coli
beta-ketoacyl-[acyl-carrier-protein] synthase I
-
Escherichia coli
KAS I
-
Escherichia coli

General Information

General Information Comment Organism
metabolism beta-ketoacyl acyl carrier protein synthase I is involved in the elongation of unsaturated fatty acids in bacterial fatty acid synthesis Escherichia coli