Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of temperature-sensitive mutation in fabB into strain K27 by phage P1-mediated transduction using closely linked Tn10 insertion as selectable marker. A fabB mutant strain accumulates less cis-5-dodecenoic acid than the parental wild-type strain. The wild-type strain also accumulates 2.7fold more cis-7-tetradecenoate than the fabB(Ts) strain. The combination of null mutations in fadD and fabA or fabB is synthetically lethal even in the presence of oleic acid supplementation. A temperature-sensitive mutation is used rather than null mutations because a strain having fabB null mutation has an obligate requirement for an unsaturated fatty acid and FabD is required for uptake of the supplement | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | the product is not incorporated into the complex lipids of the bacterium | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene fabB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | - |
? | |
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein | Escherichia coli | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | the product is not incorporated into the complex lipids of the bacterium | ? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-ACP synthase | - |
Escherichia coli |
FabB | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | FabB catalyzes the elongation of cis-3-decenoyl-ACP produced by FabA to generate the first 12-carbon cis-unsaturated fatty acid intermediate, 3-oxo-cis-5-decenoyl-ACP, which is converted to cis-5-dodecenoyl-ACP. FabB is essential in the unsaturated fatty acid, UFA, synthesis pathway, strains lacking FabB are UFA auxotroph, overview. FabB cannot be substituted by FabF | Escherichia coli |