Cloned (Comment) | Organism |
---|---|
synthases I, II, and III, recombinant expression | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
structure analysis, three-dimensional model of enzyme complexed with inhibitors thiolactomycin and cerulenin | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cerulenin | analysis of the binding mechanism, mimicks the condensation transition state; FabB: inhibition is irreversible | Escherichia coli | |
thiolactomycin | analysis of the binding mechanism, mimics malonyl-[acyl-carrier-protein]; FabB: inhibition is reversible, competitive | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
synthases I, II, and III | - |
Purification (Comment) | Organism |
---|---|
recombinant synthases I, i.e. FabA, II, i.e. FabB, and III, i.e.FabH | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | synthaseIII/FabH: His-Asn-Cys catalytic triad | Escherichia coli | |
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | synthaseII/FabB: His-HIs-Cys catalytic triad | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |