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Literature summary for 2.3.1.41 extracted from

  • Khandekar, S.S.; Gentry, D.R.; Van Aller, G.S.; Warren, P.; Xiang, H.; Silverman, C.; Doyle, M.L.; Chambers, P.A.; Konstantinidis, A.K.; Brandt, M.; Daines, R.A.; Lonsdale, J.T.
    Identification, substrate specificity, and inhibition of the Streptococcus pneumoniae beta-ketoacyl-acyl carrier protein synthase III (FabH) (2001), J. Biol. Chem., 276, 30024-30030.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tagged protein in Escherichia coli Haemophilus influenzae
overexpression of His-tagged FabH in Escherichia coli BL21 (DE3) Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
cerulenin
-
Escherichia coli
cerulenin
-
Haemophilus influenzae
cerulenin
-
Streptococcus pneumoniae
SB418011
-
Escherichia coli
SB418011
-
Haemophilus influenzae
SB418011 strong, synthase III Streptococcus pneumoniae
thiolactomycin synthase III Escherichia coli
thiolactomycin
-
Haemophilus influenzae
thiolactomycin synthase III; weak Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0186
-
malonyl-[acyl-carrier protein] synthase III Streptococcus pneumoniae
0.0403
-
acetyl-CoA synthase III Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence alignment Haemophilus influenzae
additional information
-
amino acid sequence alignment Escherichia coli
additional information
-
amino acid sequence alignment Streptococcus pneumoniae
60000
-
synthase III, gel filtration Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Haemophilus influenzae
-
-
-
Streptococcus pneumoniae P0A3C5 synthase III, gene fabH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from E. coli Haemophilus influenzae
recombinant His-tagged synthase III from E. coli Streptococcus pneumoniae

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] synthaseIII/FabH: His-Asn-Cys catalytic triad Haemophilus influenzae
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] synthaseIII/FabH: His-Asn-Cys catalytic triad Escherichia coli
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] synthaseIII/FabH: His-Asn-Cys catalytic triad Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
synthase III Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Haemophilus influenzae acetoacyl-[acyl-carrier protein] + CO2 + CoA
-
?
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Escherichia coli acetoacyl-[acyl-carrier protein] + CO2 + CoA
-
?
acetyl-CoA + malonyl-[acyl-carrier protein] synthase III, best substrate Streptococcus pneumoniae acetoacyl-[acyl-carrier protein] + CO2 + CoA
-
?
additional information
-
Escherichia coli ?
-
?
additional information synthase III, FabH, has a preference for straight short-chain CoA primers Streptococcus pneumoniae ?
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Haemophilus influenzae 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Streptococcus pneumoniae 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?