Literature summary for 2.3.1.41 extracted from

Val, D.; Banu, G.; Seshadri, K.; Lindqvist, Y.; Dehesh, K.
Re-engineering ketoacyl synthase specificity (2000), Structure, 8, 565-566.

Search for more literature for 2.3.1.41

Application

Application	Comment	Organism
medicine	target for the development of drugs for the treatment of cancer and tuberculosis	Synechocystis sp.
nutrition	target for the engineering of plant seed oils	Synechocystis sp.
pharmacology	target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents	Synechocystis sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant synthase II in Escherichia coli	Synechocystis sp.

Protein Variants

Protein Variants	Comment	Organism
A193M	site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity	Synechocystis sp.
I108F	site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein]	Synechocystis sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
cerulenin	12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity	Synechocystis sp.

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp.	-	synthase II	-

Purification (Commentary)

Purification (Comment)	Organism
synthase II, recombinant from E. coli	Synechocystis sp.

Reaction

Reaction	Comment	Organism	Reaction ID
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	active site cysteine	Synechocystis sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Synechocystis sp.	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Synechocystis sp.	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Synechocystis sp.	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
KAS	-	Synechocystis sp.

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Release 2023.1 (January 2023)