Application | Comment | Organism |
---|---|---|
medicine | target for the development of drugs for the treatment of cancer and tuberculosis | Synechocystis sp. |
nutrition | target for the engineering of plant seed oils | Synechocystis sp. |
pharmacology | target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant synthase II in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
A193M | site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity | Synechocystis sp. |
I108F | site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein] | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cerulenin | 12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
synthase II | - |
Purification (Comment) | Organism |
---|---|
synthase II, recombinant from E. coli | Synechocystis sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | active site cysteine | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KAS | - |
Synechocystis sp. |