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Literature summary for 2.3.1.41 extracted from
- beta-Ketoacyl acyl carrier protein synthase (1969), Methods Enzymol., 14, 57-60.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | required in high concentration, 0.5 M | Escherichia coli |  |
| dithiothreitol | activation | Escherichia coli | |
| dithiothreitol | can replace 2-mercaptoethanol | Escherichia coli | |
| EDTA | 7 mM, stimulates | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | after incubation with 2-mercaptethanol | Escherichia coli | |
| N-ethylmaleimide | after incubation with 2-mercaptoethanol | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
acetyl-[acyl-carrier protein] | pH 7.0 | Escherichia coli | |
| 0.025 | - |
malonyl-[acyl-carrier protein] | pH 7.0 | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | part of non-associated fatty acid synthase system of plants and prokaryotes | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | involved in biosynthesis of saturated fatty acids | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.81 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | part of non-associated fatty acid synthase system of plants and prokaryotes | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | involved in biosynthesis of saturated fatty acids | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | sharp drop in activity below pH 6.5 and above pH 8.0 | Escherichia coli |
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Release 2023.1 (January 2023)
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