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Literature summary for 2.3.1.4 extracted from

  • Ma, W.; Liu, Y.; Wang, Y.; Lv, X.; Li, J.; Du, G.; Liu, L.
    Combinatorial fine-tuning of GNA1 and GlmS expression by 5-terminus fusion engineering leads to overproduction of N-acetylglucosamine in Bacillus subtilis (2019), Biotechnol. J., 14, e1800264 .
    View publication on PubMed

Application

Application Comment Organism
synthesis overexpression in Bacillus subtilis for synthesis of N-acetylglucosamine. The expression level of GNA1 is enhanced at the translational level via fusion of an epitope tag to the 5'-terminus of GNA1 gene and ribosome binding site sequence engineering. Enhanced expression of glutamine-fructose-6-phosphate aminotransferaseGlmS is achieved at the transcriptional and translational levels. Under the control of engineered GNA1 and GlmS, the GlcNAc titer and yield in the shake flask increase to 18.5 g/l and 0.37 g GlcNAc/g glucose Caenorhabditis elegans

Cloned(Commentary)

Cloned (Comment) Organism
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Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q17427
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