Literature summary for 2.3.1.4 extracted from

Zhang, W.; Jones, V.C.; Scherman, M.S.; Mahapatra, S.; Crick, D.; Bhamidi, S.; Xin, Y.; McNeil, M.R.; Ma, Y.
Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase (2008), Int. J. Biochem. Cell Biol., 40, 2560-2571.

Search for more literature for 2.3.1.4

Application

Application	Comment	Organism
molecular biology	a microtiter plate based assay to detect the GlmU activity is developed: the assay relies on the enzymes MurA and MurB to convert UDP-GlcNAc into UDP-MurNAc with the concomitant reduction of NADPH. When all enzymes and substrates are present NADPH is oxidized with a concomitant decrease in OD340	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a GlmU knockout strain discloses that GlmU is required for growth of Mycobacterium smegmatis as the bacteria does not grow in the absence of active GlmU enzyme	Mycolicibacterium smegmatis
additional information	in a temperature shift experiment (from 30°C to 42°C) the amount of functional GlmU enzyme is gradually reduced the Mycobacterium smegmatis cells become non-viable and their morphology change from a normal rod shape to stubby-rounded morphology and in some cases they lyse	Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54100	-	SDS-PAGE	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a HIS-Select HF affinity gel column	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + D-glucosamine 6-phosphate	-	Mycolicibacterium smegmatis	CoA + N-acetyl-D-glucosamine 6-phosphate	-	?
acetyl-CoA + D-glucosamine 6-phosphate	-	Mycobacterium tuberculosis	CoA + N-acetyl-D-glucosamine 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
GlmU	GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities	Mycolicibacterium smegmatis
GlmU	GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities	Mycobacterium tuberculosis
glucosamine-6-phosphate N-acetyltransferase	-	Mycolicibacterium smegmatis
glucosamine-6-phosphate N-acetyltransferase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	assay carried out at room temperature	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)