Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GFP-fusion protein containing the mitochondrial targeting sequence in mitochondria of HeLa cells, expression of truncated enzyme forms lacking the first 21 or 59 amino acid residues, respectively, in Escherichia coli strain BL21(DE3) as soluble enzymes, or in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria, and contains a mitochondrial targeting sequence | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | Homo sapiens | absolutely specific for the substrates, type II [acyl-carrier protein] | CoA + malonyl-[acyl-carrier protein] | - |
? | |
additional information | Homo sapiens | enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated enzyme forms from Sf9 insect cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
recombinant enzyme with recombinant ACP-A from Bacillus subtilis | Homo sapiens |
0.14 | - |
recombinant enzyme with recombinant human mitochondrial [acyl-carrier protein] lacking the first 68 amino acid residues | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | absolutely specific for the substrates, type II [acyl-carrier protein] | Homo sapiens | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + [acyl-carrier protein] | absolutely specific for the substrates, recombinant type II [acyl-carrier protein] lacking the N-terminal 68 amino acid residues from human, or recombinant ACP-A from Bacillus subtilis | Homo sapiens | CoA + malonyl-[acyl-carrier protein] | - |
? | |
additional information | enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Homo sapiens |