Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
in complex with pyridoxal 5'-phosphate, using 0.1 M tri-sodium citrate (pH 5.5), 20% (w/v) PEG 3000 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTACT | the variant lacking residues 535-548 has reduced activity (about 65% of wild type) | Saccharomyces cerevisiae |
R151A | the mutant shows 20% of wild type activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Saccharomyces cerevisiae | - |
5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P09950 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose resin column chromatography and Superdex S200 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Saccharomyces cerevisiae | 5-aminolevulinate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
5-aminolevulinic acid synthase | - |
Saccharomyces cerevisiae |
ALAS | - |
Saccharomyces cerevisiae |
Hem1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | one enzyme subunit contains covalently bound pyridoxal 5'-phosphate whereas the second is pyridoxal 5'-phosphate-free | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first step in heme biosynthesis | Saccharomyces cerevisiae |