Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Gold (DE3) cells | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
T363S | the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme | Cereibacter sphaeroides |
T83S | the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme | Cereibacter sphaeroides |
T83S/T363S | the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0146 | - |
succinyl-CoA | wild type enzyme, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.0774 | - |
succinyl-CoA | mutant enzyme T83S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.1302 | - |
succinyl-CoA | mutant enzyme T83S/T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.1645 | - |
succinyl-CoA | mutant enzyme T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
11.3 | - |
glycine | mutant enzyme T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
14 | - |
glycine | mutant enzyme T83S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
16.7 | - |
glycine | mutant enzyme T83S/T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
31.7 | - |
glycine | wild type enzyme, at pH 7.2 and 37°C | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Cereibacter sphaeroides | - |
5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni superflow resin column chromatography | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinate-CoA | weak cyclization activity | Cereibacter sphaeroides | 2-amino-3-hydroxycyclopent-2-en-1-one + CoASH | - |
- |
|
succinyl-CoA + glycine | - |
Cereibacter sphaeroides | 5-aminolevulinate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * around 50000, SDS-PAGE | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
ALA synthase | - |
Cereibacter sphaeroides |
hemA | - |
Cereibacter sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
glycine | mutant enzyme T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
12.1 | - |
glycine | mutant enzyme T83S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
13.1 | - |
glycine | mutant enzyme T83S/T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
63.4 | - |
glycine | wild type enzyme, at pH 7.2 and 37°C | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Cereibacter sphaeroides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
succinyl-CoA | mutant enzyme T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.102 | - |
succinyl-CoA | mutant enzyme T83S/T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.16 | - |
succinyl-CoA | mutant enzyme T83S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.79 | - |
glycine | mutant enzyme T83S/T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
0.87 | - |
glycine | mutant enzyme T83S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
1.07 | - |
glycine | mutant enzyme T363S, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
2 | - |
glycine | wild type enzyme, at pH 7.2 and 37°C | Cereibacter sphaeroides | |
4.34 | - |
succinyl-CoA | wild type enzyme, at pH 7.2 and 37°C | Cereibacter sphaeroides |