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Literature summary for 2.3.1.37 extracted from

  • Liu, J.; Kaganjo, J.; Zhang, W.; Zeilstra-Ryalls, J.
    Investigating the bifunctionality of cyclizing and classical 5-aminolevulinate synthases (2018), Protein Sci., 27, 402-410 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Gold (DE3) cells Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
T363S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides
T83S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides
T83S/T363S the mutant shows strongly reduced 5-aminolevulinate synthase activity relative to the wild type enzyme Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0146
-
succinyl-CoA wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides
0.0774
-
succinyl-CoA mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.1302
-
succinyl-CoA mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.1645
-
succinyl-CoA mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
11.3
-
glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
14
-
glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
16.7
-
glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
31.7
-
glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Cereibacter sphaeroides
-
5-aminolevulinate + CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni superflow resin column chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminolevulinate-CoA weak cyclization activity Cereibacter sphaeroides 2-amino-3-hydroxycyclopent-2-en-1-one + CoASH
-
-
succinyl-CoA + glycine
-
Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
?

Subunits

Subunits Comment Organism
? x * around 50000, SDS-PAGE Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
ALA synthase
-
Cereibacter sphaeroides
hemA
-
Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
12.1
-
glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
13.1
-
glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
63.4
-
glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Cereibacter sphaeroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.073
-
succinyl-CoA mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.102
-
succinyl-CoA mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.16
-
succinyl-CoA mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.79
-
glycine mutant enzyme T83S/T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
0.87
-
glycine mutant enzyme T83S, at pH 7.2 and 37°C Cereibacter sphaeroides
1.07
-
glycine mutant enzyme T363S, at pH 7.2 and 37°C Cereibacter sphaeroides
2
-
glycine wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides
4.34
-
succinyl-CoA wild type enzyme, at pH 7.2 and 37°C Cereibacter sphaeroides