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Literature summary for 2.3.1.37 extracted from

  • Turbeville, T.D.; Zhang, J.; Adams, W.C.; Hunter, G.A.; Ferreira, G.C.
    Functional asymmetry for the active sites of linked 5-aminolevulinate synthase and 8-amino-7-oxononanoate synthase (2011), Arch. Biochem. Biophys., 511, 107-117.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information a single chain dimeric ALAS variant is created, in which one of the two active sites harbors a K313A mutation eliminating measurable enzyme activity in order to investigate the unusual enhanced enzymatic activity resulting from linking ALAS dimmers. The two active sites in ALAS/ALAS differentially contribute to the enhanced activity of the enzyme, even though the amount of ALA produced during the first turnover is identical in both active sites. The kcat values of the K313A variants differ significantly depending on which of the two active sites harbors the mutation Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 glycine pH 7.5, 30°C, ALAS(K313A mutant)/ALAS Mus musculus
0.00045
-
 glycine pH 7.5, 30°C, ALAS/ALAS Mus musculus
0.0018
-
 glycine pH 7.5, 30°C, ALAS/ALAS (K313A mutant) Mus musculus
0.002
-
 glycine pH 7.5, 30°C, ALAS Mus musculus
2 3 glycine pH 7.5, 30°C, ALAS Mus musculus
11.8
-
 glycine pH 7.5, 30°C, ALAS/ALAS (K313A mutant) Mus musculus
14.8
-
 glycine pH 7.5, 30°C, ALAS(K313A mutant)/ALAS Mus musculus
16.7
-
 glycine pH 7.5, 30°C, ALAS/ALAS Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Synonyms

Synonyms Comment Organism
5-aminolevulinate synthase
-
 Mus musculus
ALAS
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 glycine pH 7.5, 30°C, ALAS/ALAS (K313A mutant) Mus musculus
0.166
-
 glycine pH 7.5, 30°C, ALAS Mus musculus
0.36
-
 glycine pH 7.5, 30°C, ALAS(K313A mutant)/ALAS Mus musculus
0.92
-
 glycine pH 7.5, 30°C, ALAS/ALAS Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus