Literature summary for 2.3.1.32 extracted from

Ko, M.H.; Puglielli, L.
Two endoplasmic reticulum (ER)/ER intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels (2009), J. Biol. Chem., 284, 2482-2492.

Activating Compound

Activating Compound	Comment	Organism	Structure
membrane transporter	for acetyl-CoA and acetyltransferase from cytosol into lumen of the ER/ER Golgi intermediate compartment	Homo sapiens

Application

Application	Comment	Organism
medicine	pathogenesis of late-onset Alzheimer disease, post-translational regulation of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels, a membrane protein that acts as the rate-limiting enzyme in the generation of Alzheimer disease amyloid beta-peptide, acetylation protect the protein from degradation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
PCR-amplification, stable transfection of CHO cells, with myc-tag, overexpressing enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
siRNA	silencing of enzyme gene	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum membrane	catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC)	Homo sapiens	5789	-
Golgi membrane	catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC)	Homo sapiens	139	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	SDS-PAGE, rough estimation from figure	Homo sapiens
28000	-	SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1	Homo sapiens	-	CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UHF3	-	-

Purification (Commentary)

Purification (Comment)	Organism
cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation	Homo sapiens

Purification (Comment)

Organism

cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation

Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
H4 neuroglioma cell	-	Homo sapiens	-
SH-SY5Y cell	neuroblastoma	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1	-	Homo sapiens	CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1	-	?
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1	50 mM Tris-HCl, pH 8.0, 30°C	Homo sapiens	CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1	-	?
acetyl-CoA + histone H3 N-terminal tail	50 mM Tris-HCl, pH 8.0, 30°C	Homo sapiens	CoA + acetylated histone H3 N-terminal tail	-	?

Synonyms

Synonyms	Comment	Organism
ATase1	-	Homo sapiens
ATase2	-	Homo sapiens
lysine acetyltransferase	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	transfected cells increase acetyltransferase activity 2fold, and by the treatment with the lipid second messenger ceramide	up
Homo sapiens	transfected cells increase acetyltransferase activity 3fold, and by the treatment with the lipid second messenger ceramide	up

General Information

General Information	Comment	Organism
metabolism	beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC)	Homo sapiens