Cloned (Comment) | Organism |
---|---|
expression in Pseudomonas stutzeri | Geobacter metallireducens |
Crystallization (Comment) | Organism |
---|---|
structure of BbsAB with and without bound CoA at is determined at 1.7 A and 2.0 A resolution | Geobacter metallireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.114 | - |
benzoyl-CoA | calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication | Geobacter metallireducens | |
0.31 | - |
succinyl-CoA | calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication | Geobacter metallireducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
BbsA, gel filtration | Geobacter metallireducens |
91000 | - |
BbsB, gel filtration | Geobacter metallireducens |
125000 | - |
holoenzyme, gel filtration | Geobacter metallireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-benzoylsuccinyl-CoA + CoA | Geobacter metallireducens | the enzyme catalyzes the final step of anaerobic toluene metabolism | benzoyl-CoA + succinyl-CoA | - |
r | |
(S)-2-benzoylsuccinyl-CoA + CoA | Geobacter metallireducens ATCC 53774 | the enzyme catalyzes the final step of anaerobic toluene metabolism | benzoyl-CoA + succinyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter metallireducens | Q39VG2 AND Q39VG1 | Q39VG2: bbsA, Q39VG1: bbsB | - |
Geobacter metallireducens ATCC 53774 | Q39VG2 AND Q39VG1 | Q39VG2: bbsA, Q39VG1: bbsB | - |
Purification (Comment) | Organism |
---|---|
the BbsAB complex is purified in one step via strep-tag affinity chromatography without apparent splitting up into the respective subunits, yielding 50-60 mg pure protein per 10 g wet cell mass, further purified by size exclusion chromatography | Geobacter metallireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-benzoylsuccinyl-CoA + CoA | the enzyme catalyzes the final step of anaerobic toluene metabolism | Geobacter metallireducens | benzoyl-CoA + succinyl-CoA | - |
r | |
(S)-2-benzoylsuccinyl-CoA + CoA | thioester-dependent Claisen-type mechanism | Geobacter metallireducens | benzoyl-CoA + succinyl-CoA | - |
r | |
(S)-2-benzoylsuccinyl-CoA + CoA | the enzyme catalyzes the final step of anaerobic toluene metabolism | Geobacter metallireducens ATCC 53774 | benzoyl-CoA + succinyl-CoA | - |
r | |
(S)-2-benzoylsuccinyl-CoA + CoA | thioester-dependent Claisen-type mechanism | Geobacter metallireducens ATCC 53774 | benzoyl-CoA + succinyl-CoA | - |
r | |
benzoyl-CoA + succinyl-CoA | - |
Geobacter metallireducens | (S)-2-benzoylsuccinyl-CoA + CoA | - |
r | |
benzoyl-CoA + succinyl-CoA | - |
Geobacter metallireducens ATCC 53774 | (S)-2-benzoylsuccinyl-CoA + CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
(S)-2-benzoylsuccinyl-CoA thiolase | - |
Geobacter metallireducens |
bbsAB | - |
Geobacter metallireducens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the final step of anaerobic toluene metabolism | Geobacter metallireducens |