Cloned (Comment) | Organism |
---|---|
met2 gene, expression in Escherichia coli BL21 (DE3) | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
propionyl-CoA | - |
Haemophilus influenzae | |
0.13 | - |
L-homoserine | - |
Haemophilus influenzae | |
0.13 | - |
crotonyl-CoA | - |
Haemophilus influenzae | |
0.14 | - |
acetyl-CoA | - |
Haemophilus influenzae | |
0.21 | - |
butyryl-CoA | - |
Haemophilus influenzae | |
0.28 | - |
glutaryl-CoA | - |
Haemophilus influenzae | |
0.36 | - |
succinyl-CoA | - |
Haemophilus influenzae | |
0.62 | - |
CoA | - |
Haemophilus influenzae | |
0.85 | - |
O-acetyl-L-homoserine | - |
Haemophilus influenzae | |
1.4 | - |
4-nitrophenyl acetate | - |
Haemophilus influenzae | |
4.7 | - |
D-homoserine | - |
Haemophilus influenzae | |
19 | - |
4-hydroxybutyric acid | - |
Haemophilus influenzae | |
71 | - |
3-Amino-1-propanol | - |
Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
gel filtration | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-homoserine | Haemophilus influenzae | control point in the pathway | CoA + O-acetyl-L-homoserine | - |
r | |
acetyl-CoA + L-homoserine | Haemophilus influenzae | first reaction in L-methionine biosynthesis | CoA + O-acetyl-L-homoserine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
MET2 gene | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Haemophilus influenzae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine | ping-pong reaction mechanism | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-amino-1-propanol | low activity | Haemophilus influenzae | ? | - |
r | |
acetyl-CoA + 4-nitrophenyl acetate | low activity | Haemophilus influenzae | ? | - |
r | |
acetyl-CoA + D-homoserine | - |
Haemophilus influenzae | CoA + O-acetyl-D-homoserine | - |
r | |
acetyl-CoA + gamma-hydroxybutyric acid | - |
Haemophilus influenzae | ? | - |
r | |
acetyl-CoA + L-homoserine | preference for acetyl-CoA | Haemophilus influenzae | CoA + O-acetyl-L-homoserine | - |
r | |
acetyl-CoA + L-homoserine | control point in the pathway | Haemophilus influenzae | CoA + O-acetyl-L-homoserine | - |
r | |
acetyl-CoA + L-homoserine | first reaction in L-methionine biosynthesis | Haemophilus influenzae | CoA + O-acetyl-L-homoserine | - |
r | |
butyryl-CoA + L-homoserine | - |
Haemophilus influenzae | CoA + O-butyryl-L-homoserine | - |
? | |
crotonyl-CoA + L-homoserine | low activity | Haemophilus influenzae | CoA + O-crotonyl-L-homoserine | - |
r | |
glutaryl-CoA + L-homoserine | very low activity | Haemophilus influenzae | CoA + O-glutaryl-L-homoserine | - |
r | |
propionyl-CoA + L-homoserine | - |
Haemophilus influenzae | CoA + O-propionyl-L-homoserine | - |
? | |
succinyl-CoA + L-homoserine | very low activity | Haemophilus influenzae | CoA + O-succinyl-L-homoserine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000-40000, SDS-PAGE and electrospray ionization-mass spectrometry | Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
3-Amino-1-propanol | - |
Haemophilus influenzae | |
7.8 | - |
4-nitrophenyl acetate | - |
Haemophilus influenzae | |
15 | - |
crotonyl-CoA | - |
Haemophilus influenzae | |
27 | - |
butyryl-CoA | - |
Haemophilus influenzae | |
30 | - |
propionyl-CoA | - |
Haemophilus influenzae | |
31 | - |
O-acetyl-L-homoserine | - |
Haemophilus influenzae | |
50 | - |
4-hydroxybutyric acid | - |
Haemophilus influenzae | |
78 | - |
D-homoserine | - |
Haemophilus influenzae | |
92 | - |
acetyl-CoA | - |
Haemophilus influenzae | |
92 | - |
L-homoserine | - |
Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Haemophilus influenzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Haemophilus influenzae |