Protein Variants | Comment | Organism |
---|---|---|
additional information | methionine auxotroph strains show no activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-Penicillamine | 3.3 mM, complete inhibition | Saccharomyces cerevisiae | |
L-cysteine | weak | Saccharomyces cerevisiae | |
L-homocysteine | - |
Saccharomyces cerevisiae | |
L-methionine | no inhibition; repression of enzyme expression | Saccharomyces cerevisiae | |
additional information | no inhibition by MgCl2, MnCl2, ZnCl2, hydroxylamine, phenylhydrazine, D-homoserine, methionine, L-tyrosine, L-serine and nucleotides | Saccharomyces cerevisiae | |
O-acetyl-L-homoserine | - |
Saccharomyces cerevisiae | |
O-acetyl-L-serine | weak | Saccharomyces cerevisiae | |
O-phospho-L-homoserine | weak | Saccharomyces cerevisiae | |
O-succinyl-L-homoserine | weak | Saccharomyces cerevisiae | |
S-adenosyl-L-homocysteine | weak | Saccharomyces cerevisiae | |
S-adenosyl-L-methionine | weak | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
acetyl-CoA | deacylation | Saccharomyces cerevisiae | |
0.027 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
1 | - |
L-homoserine | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | 104000 | sucrose density gradient centrifugation, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
haploid wild-type strain (a-mating type) and met-auxotroph mutant strains 6,8,12,13,15, and 17 | - |
Purification (Comment) | Organism |
---|---|
partial, mutant strain 6 | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.4 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-homoserine | succinyl-CoA cannot replace acetyl-CoA | Saccharomyces cerevisiae | CoA + O-acetyl-L-homoserine | - |
ir | |
acetyl-CoA + L-homoserine | not D-homoserine or any other hydroxy-L-amino acid | Saccharomyces cerevisiae | CoA + O-acetyl-L-homoserine | - |
ir | |
additional information | deacylates acetyl-CoA in the absence of L-homoserine | Saccharomyces cerevisiae | ? | - |
? | |
additional information | catalyzes reversible L-homoserine/O-acetyl-L-homoserine exchange in the absence of acetyl-CoA | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.0 | Saccharomyces cerevisiae |
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
most stable | Saccharomyces cerevisiae |