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Literature summary for 2.3.1.304 extracted from

  • Satoh, K.; Kawakami, T.; Isobe, N.; Pasquier, L.; Tomita, H.; Zinn, M.; Matsumoto, K.
    Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR (2022), Microb. Cell Fact., 21, 84.
    View publication on PubMed
Search for more literature for 2.3.1.304

Protein Variants

Protein Variants Comment Organism
additional information sequence-regulating polyhydroxyalkanoate synthase PhaCAR is a chimeric enzyme comprising PhaCs from Aeromonas caviae and Ralstonia eutropha (Cupriavidus necator). Generation and evaluation of an versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR, overview Aeromonas caviae
additional information sequence-regulating polyhydroxyalkanoate synthase PhaCAR is a chimeric enzyme comprising PhaCs from Aeromonas caviae and Ralstonia eutropha (Cupriavidus necator). Generation and evaluation of an versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR, overview Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Aeromonas caviae
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Cupriavidus necator
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Cupriavidus necator DSM 428
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Aeromonas caviae O32471
-
-
Cupriavidus necator P23608
-
-
Cupriavidus necator DSM 428 P23608
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Aeromonas caviae CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Cupriavidus necator CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
(3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Cupriavidus necator DSM 428 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
(R,S)-2-hydroxybutyryl-CoA + glycolate-CoA polymerization of GL-CoA and 3HB-CoA Aeromonas caviae CoA + poly[2-hydroxybutyrate (3HB)]-beta-poly[glycolate (GL)-ran-3HB]
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 3-hydroxypropionate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-3-hydroxypropionate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 4-hydroxy-2-methylbutyrate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-4-hydroxy-2-methylbutyrate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + 5-hydroxyvalerate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-5-hydroxyvalerate) + CoA
-
 ?
(R,S)-3-hydroxybutyryl-CoA + glycolate-CoA polymerization of GL-CoA and 3HB-CoA Cupriavidus necator CoA + poly[3-hydroxybutyrate (3HB)]-beta-poly[glycolate (GL)-ran-3HB]
-
 ?
(R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Aeromonas caviae poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
(R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Cupriavidus necator poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
(R,S)-3-hydroxypropionyl-CoA + 6-hydroxyhexanoate
-
 Cupriavidus necator DSM 428 poly(3-hydroxybutyrate-co-6-hydroxyhexanoate) + CoA
-
 ?
additional information synthesis of 3HB-based binary copolymers containing 3HP, 4H2MB, 5HV, and 6HHx as secondary monomer units by recombinant chimeric enzyme PhaCAR in Escherichia coli. PhaCAR can efficiently incorporate 2HB and GL units into polymers. Using this enzyme, PHA block copolymers, P(2HB)-b-(3HB), P(GL-ran-3HB)-b-P(3HB), and P(GL-co-3HBco-3HHx)-b-P(3HB-co-3HHx) are synthesized. 2HB-containing ternary copolymer production is conducted using 3HB, 3HP, and LMC HAs in combination. The enzyme shows preference of PhaCAR for 3HB and 3HP monomers. Substrate specificity of chimeric enzyme PhaCAR, detailed overview Aeromonas caviae ?
-
-
additional information synthesis of 3HB-based binary copolymers containing 3HP, 4H2MB, 5HV, and 6HHx as secondary monomer units by recombinant chimeric enzyme PhaCAR in Escherichia coli. PhaCAR can efficiently incorporate 2HB and GL units into polymers. Using this enzyme, PHA block copolymers, P(2HB)-b-(3HB), P(GL-ran-3HB)-b-P(3HB), and P(GL-co-3HBco-3HHx)-b-P(3HB-co-3HHx) are synthesized. 2HB-containing ternary copolymer production is conducted using 3HB, 3HP, and LMC HAs in combination. The enzyme shows preference of PhaCAR for 3HB and 3HP monomers. Substrate specificity of chimeric enzyme PhaCAR, detailed overview Cupriavidus necator ?
-
-

Synonyms

Synonyms Comment Organism
class I 2HA-incorporating PHA synthase
-
 Aeromonas caviae
class I 2HA-incorporating PHA synthase
-
 Cupriavidus necator
PhaCAR
-
 Aeromonas caviae
PhaCAR
-
 Cupriavidus necator
sequence-regulating polyhydroxyalkanoate synthase PhaCAR
-
 Aeromonas caviae
sequence-regulating polyhydroxyalkanoate synthase PhaCAR
-
 Cupriavidus necator

General Information

General Information Comment Organism
physiological function PhaCAR is a class I 2HA-incorporating PHA synthase. Class I PHA synthases can recognize unusual long-main-chain hydroxyalkanoates (LMC HAs, C4 or more in the main-chain) monomers, such as 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) Aeromonas caviae
physiological function PhaCAR is a class I 2HA-incorporating PHA synthase. Class I PHA synthases can recognize unusual long-main-chain hydroxyalkanoates (LMC HAs, C4 or more in the main-chain) monomers, such as 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) Cupriavidus necator