Literature summary for 2.3.1.304 extracted from

Chek, M.F.; Kim, S.Y.; Mori, T.; Arsad, H.; Samian, M.R.; Sudesh, K.; Hakoshima, T.
Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics (2017), Sci. Rep., 7, 5312 .

Search for more literature for 2.3.1.304

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells	Chromobacterium sp. USM2

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic domain, vapor diffusion method, using 50 mM Bis-Tris (pH 5.5), 60 mM ammonium sulfate and 5% (w/v) PEG4000. Full-length enzyme, vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Bis-Tris (pH 6.5) and 25% (w/v) PEG3350	Chromobacterium sp. USM2

Organism

Organism	UniProt	Comment	Textmining
Chromobacterium sp. USM2	E1APK1	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q anion-exchange column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration	Chromobacterium sp. USM2

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-3-hydroxbutyryl-CoA + poly[DL-3-hydroxybutyrate]n	-	Chromobacterium sp. USM2	CoA + poly[DL-3-hydroxybutyrate]n+1	-	?

Subunits

Subunits	Comment	Organism
?	x * 43000, catalytic domain, SDS-PAGE	Chromobacterium sp. USM2
?	x * 63000, full-length enzyme, SDS-PAGE	Chromobacterium sp. USM2

Synonyms

Synonyms	Comment	Organism
PHA synthase	-	Chromobacterium sp. USM2
PhaC	-	Chromobacterium sp. USM2
polyhydroxyalkanoate synthase	-	Chromobacterium sp. USM2

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Release 2023.1 (January 2023)