Literature summary for 2.3.1.304 extracted from

Shen, X.W.; Shi, Z.Y.; Song, G.; Li, Z.J.; Chen, G.Q.
Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers (2011), Appl. Microbiol. Biotechnol., 91, 655-665 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Ralstonia eutropha PHB-4	Pseudomonas stutzeri

Protein Variants

Protein Variants	Comment	Organism
S326T/Q482K	the mutations increase the enzyme substrate specificity toward 3-hydroxybutyrate enhance synthase activity for more PHA production	Pseudomonas stutzeri

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas stutzeri	-	-	-
Pseudomonas stutzeri 1317	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n	-	Pseudomonas stutzeri	CoA + poly[(R)-3-hydroxybutyrate]n+1	-	?
(3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n	-	Pseudomonas stutzeri 1317	CoA + poly[(R)-3-hydroxybutyrate]n+1	-	?

Synonyms

Synonyms	Comment	Organism
PHA synthase	-	Pseudomonas stutzeri
PhaC2Ps	-	Pseudomonas stutzeri
polyhydroxyalkanoate synthase	-	Pseudomonas stutzeri

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Release 2023.1 (January 2023)