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Literature summary for 2.3.1.304 extracted from

  • Nguyen, T.H.; Ishizuna, F.; Sato, Y.; Arai, H.; Ishii, M.
    Physiological characterization of poly-beta-hydroxybutyrate accumulation in the moderately thermophilic hydrogen-oxidizing bacterium Hydrogenophilus thermoluteolus TH-1 (2019), J. Biosci. Bioeng., 127, 686-689 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene phaC, sequence comparisons, phylogenetic analysis Hydrogenophilus thermoluteolus

Organism

Organism UniProt Comment Textmining
Hydrogenophilus thermoluteolus A0A2Z6DVS4
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Hydrogenophilus thermoluteolus A0A2Z6DWT1
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Hydrogenophilus thermoluteolus DSM 6765 A0A2Z6DVS4
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Hydrogenophilus thermoluteolus DSM 6765 A0A2Z6DWT1
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Hydrogenophilus thermoluteolus TH-1 A0A2Z6DVS4
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Hydrogenophilus thermoluteolus TH-1 A0A2Z6DWT1
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Source Tissue

Source Tissue Comment Organism Textmining
additional information two-phase culture of strain TH-1 for PHB production under autotrophic and heterotrophic condition, overview Hydrogenophilus thermoluteolus
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Synonyms

Synonyms Comment Organism
Class I poly(R)-hydroxyalkanoic acid synthase UniProt Hydrogenophilus thermoluteolus
class I polyhydroxyalkanoate synthase
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 Hydrogenophilus thermoluteolus
HPTL0263 locus name Hydrogenophilus thermoluteolus
HPTL0635 locus name Hydrogenophilus thermoluteolus
HPTL1376 locus name Hydrogenophilus thermoluteolus
PHB synthase
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 Hydrogenophilus thermoluteolus
PhbC
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 Hydrogenophilus thermoluteolus
poly-beta-hydroxybutyrate synthase UniProt Hydrogenophilus thermoluteolus
polyhydroxyalkanoate synthase
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 Hydrogenophilus thermoluteolus
TH-1_PHB synthase_HPTL_0263
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 Hydrogenophilus thermoluteolus
TH-1_PHB synthase_HPTL_0635
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 Hydrogenophilus thermoluteolus
TH-1_PHB synthase_HPTL_1376
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 Hydrogenophilus thermoluteolus

Expression

Organism Comment Expression
Hydrogenophilus thermoluteolus poly-beta-hydroxybutyrate (PHB) accumulation is induced by nitrogen limitation under autotrophic as well as heterotrophic conditions up

General Information

General Information Comment Organism
metabolism Hydrogenophilus thermoluteolus strain TH-1 is a thermophilic hydrogen-oxidizing microorganism that has the highest growth rate among autotrophs. Genomic analysis reveals that this strain comprises the complete gene set for poly-beta-hydroxybutyrate (PHB) synthesis, i.e. three copies of acetyl-CoA acetyltransferase and polyhydroxyalkanoate synthase and one copy of acetoacetyl-CoA reductase and 3-hydroxyacyl-CoA dehydrogenase/3-hydroxybutyryl-CoA epimerase. PHB accumulation is induced by nitrogen limitation under autotrophic as well as heterotrophic conditions. This strain accumulates up to 430.4 mg LL1 PHB during a 3-h incubation under nitrogen-limited heterotrophic conditions. The highest PHB accumulation rates under autotrophic and heterotrophic conditions are 38.6% (w/w) of the dry cells after a 6-h induction and 53.8% after 3 h, respectively. Although PHB granules start to accumulate after 15 min of nitrogen limitation under heterotrophic conditions, a drastic decrease of PHB is observed after 9 h of induction. Among these synthetic genes, polyhydroxyalkanoate synthase (phbC) is the key enzyme for the polymerization Hydrogenophilus thermoluteolus
metabolism Hydrogenophilus thermoluteolus strain TH-1 is a thermophilic hydrogen-oxidizing microorganism that has the highest growth rate among autotrophs. Genomic analysis reveals that this strain comprises the complete gene set for poly-beta-hydroxybutyrate (PHB) synthesis, i.e. three copies of acetyl-CoA acetyltransferase and polyhydroxyalkanoate synthase and one copy of acetoacetyl-CoA reductase and 3-hydroxyacyl-CoA dehydrogenase/3-hydroxybutyryl-CoA epimerase. PHB accumulation is induced by nitrogen limitation under autotrophic as well as heterotrophic conditions. This strain accumulates up to 430.4 mg LL1 PHB during a 3-h incubation under nitrogen-limited heterotrophic conditions. The highest PHB accumulation rates under autotrophic and heterotrophic conditions are 38.6% w/w of the dry cells after a 6-h induction and 53.8% after 3 h, respectively. Although PHB granules start to accumulate after 15 min of nitrogen limitation under heterotrophic conditions, a drastic decrease of PHB is observed after 9 h of induction. Among these synthetic genes, polyhydroxyalkanoate synthase (phbC) is the key enzyme for the polymerization Hydrogenophilus thermoluteolus
physiological function poly-beta-hydroxybutyrate accumulation in the moderately thermophilic hydrogen-oxidizing bacterium Hydrogenophilus thermoluteolus TH-1 by induction of the poly-beta-hydroxybutyrate (PHB) synthesis pathway enzymes, including the PHB synthase. Among these synthetic genes, polyhydroxyalkanoate synthase (phbC) is the key enzyme for the polymerization Hydrogenophilus thermoluteolus