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Literature summary for 2.3.1.304 extracted from

  • Chen, Y.J.; Tsai, P.C.; Hsu, C.H.; Lee, C.Y.
    Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate (2014), Enzyme Microb. Technol., 56, 60-66.
    View publication on PubMed
Search for more literature for 2.3.1.304

Application

Application Comment Organism
synthesis expansion of the substrate specificity and enhancment of biosynthesis of polyhydroxyalkanoate by site-specific mutagenesis. Mutated PhaC1s are coexpressed with beta-ketothiolase and acetoacetyl-CoA reductase to supply sufficient short-chain length (R)-3-hydroxyacyl-CoA as a substrate. Mutation L484V remarkably enhances the monomer ratio of (R)-3-hydroxybutyrate in a polyhydroxyalkanoate accumulation experiment. Val is the most favorable amino acid for incorporating (R)-3-hydroxybutyrate unit synthesis. A single mutation at Q481M, S482G and A547V obviously increases polyhydroxyalkanoate yields. Q481M and S482G enhance the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pseudomonas putida GPp104 PHA mutant Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
A547V mutation increases polyhydroxyalkanoate yields Pseudomonas putida
L484V mutation remarkably enhances the monomer ratio of (R)-3-hydroxybutyrate in a polyhydroxyalkanoate accumulation experiment. Val is the most favorable amino acid for incorporating (R)-3-hydroxybutyrate unit synthesis Pseudomonas putida
Q481M mutation increases polyhydroxyalkanoate yields and enhances the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida
S482G mutation increases polyhydroxyalkanoate yields and enhances the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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-
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Pseudomonas putida GPo1 / ATCC29347
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Synonyms

Synonyms Comment Organism
PhaC1
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 Pseudomonas putida