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Literature summary for 2.3.1.304 extracted from

  • Hyakutake, M.; Tomizawa, S.; Mizuno, K.; Abe, H.; Tsuge, T.
    Alcoholytic cleavage of polyhydroxyalkanoate chains by class IV synthases induced by endogenous and exogenous ethanol (2014), Appl. Environ. Microbiol., 80, 1421-1429.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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Bacillus cereus YB-4 D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
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 Bacillus cereus [(R)-3-hydroxybutanoate](n+1) + CoA
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 ?
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
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 Bacillus cereus YB-4 [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
additional information enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol Bacillus cereus ?
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 ?
additional information enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol Bacillus cereus YB-4 ?
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 ?

General Information

General Information Comment Organism
physiological function recombinant Escherichia coli expressing PHA synthase from Bacillus cereus shows a reduction of the molecular weight of PHA produced during the stationary phase of growth. Its carboxy end structure is capped by ethanol, as the result of alcoholytic cleavage of PHA chains by PhaRC induced by endogenous ethanol. This scission reaction is also induced by exogenous ethanol in both in vivo and in vitro assays. In addition, PhaRC has alcoholysis activity for PHA chains synthesized by other synthases Bacillus cereus