Literature summary for 2.3.1.304 extracted from

Tan, Y.; Neo, P.C.; Najimudin, N.; Sudesh, K.; Muhammad, T.S.; Othman, A.S.; Samian, R.
Cloning and characterization of poly(3-hydroxybutyrate) biosynthesis genes from Pseudomonas sp. USM 4-55 (2010), J. Basic Microbiol., 50, 179-189.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene phbCPs encoded in the phb operon, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the genetic organization of phb operon shows a putative promoter region, followed by phbBPs-phbAPs-phbCPs, with phbRPs encoding a putative transcriptional activator that is located in the opposite orientation, upstream of phbBACPs. Heterologous expression of phbCPs from pGEM3ABex vector in Escherichia coli JM109 resulting in P(3HB) accumulation of up to 40% of dry cell weight	Pseudomonas sp.

Cloned (Comment)

Organism

gene phbCPs encoded in the phb operon, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the genetic organization of phb operon shows a putative promoter region, followed by phbBPs-phbAPs-phbCPs, with phbRPs encoding a putative transcriptional activator that is located in the opposite orientation, upstream of phbBACPs. Heterologous expression of phbCPs from pGEM3ABex vector in Escherichia coli JM109 resulting in P(3HB) accumulation of up to 40% of dry cell weight

Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62300	-	x * 62300, about, sequence calculation	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	C0KTZ0	isolated in Malaysia, gene phbC encoded in the phb operon	-
Pseudomonas sp. USM4-55	C0KTZ0	isolated in Malaysia, gene phbC encoded in the phb operon	-

Subunits

Subunits	Comment	Organism
?	x * 62300, about, sequence calculation	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
P(3HB) synthase	-	Pseudomonas sp.
PhbC	-	Pseudomonas sp.

General Information

General Information	Comment	Organism
physiological function	P(3HB) synthase catalyzes polymerization of the 3-hydroxybutyryl-CoA monomers, Pseudomonas sp. USM 4-55 is a soil isolated bacterium that possesses the ability to produce polyhydroxyalkanoates consisting of both poly(3-hydroxybutyrate) homopolymer and medium-chain length monomers (6 to 14 carbon atoms) when sugars or fatty acids are utilized as the sole carbon source	Pseudomonas sp.