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Literature summary for 2.3.1.304 extracted from

  • Tomizawa, S.; Hyakutake, M.; Saito, Y.; Agus, J.; Mizuno, K.; Abe, H.; Tsuge, T.
    Molecular weight change of polyhydroxyalkanoate (PHA) caused by the PhaC subunit of PHA synthase from Bacillus cereus YB-4 in recombinant Escherichia coli (2011), Biomacromolecules, 12, 2660-2666.
    View publication on PubMed
Search for more literature for 2.3.1.304

Cloned(Commentary)

Cloned (Comment) Organism
expression of PhaRCBm, and as hybrid enzyme PhaRYB4CBm with Bacillus cereus YB-4, PhaRCYB4 in Escherichia coli JM109 Priestia megaterium
expression of PhaRCYB4, and as hybrid enzyme PhaRYB4CBm with Bacillus megaterium NBRC15308, PhaRCBm in Escherichia coli JM109 Bacillus cereus

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme hybrids of PhaC from Bacillus megaterium and Bacillus cereus, i.e. PhaRBmCYB4 and PhaRYB4CBm. The molecular weight of P(3HB) synthesized by PhaRCYB4 decreased with increasing culture time and temperature, time-dependent behavior is observed for hybrid synthase PhaRBmCYB4, but not for PhaRYB4CBm, thus the molecular weight change is caused by the PhaCYB4 subunit Priestia megaterium
additional information construction of enzyme hybrids of PhaC from Bacillus megaterium and Bacillus cereus, i.e. PhaRBmCYB4 and PhaRYB4CBm. The molecular weight of P(3HB) synthesized by PhaRCYB4 decreased with increasing culture time and temperature, time-dependent behavior is observed for hybrid synthase PhaRBmCYB4, but not for PhaRYB4CBm, thus the molecular weight change is caused by the PhaCYB4 subunit Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n Bacillus cereus PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n Priestia megaterium PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n Bacillus cereus YB-4 PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n Priestia megaterium NBRC15308 PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
additional information Bacillus cereus the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview ?
-
 ?
additional information Bacillus cereus YB-4 the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus D2Z0B8 isolated as a PHA producer from the polluted soil in Kitakyushu City, Japan
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Bacillus cereus YB-4 D2Z0B8 isolated as a PHA producer from the polluted soil in Kitakyushu City, Japan
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Priestia megaterium D2Z0B9
-
-
Priestia megaterium NBRC15308 D2Z0B9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Priestia megaterium [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Bacillus cereus [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 Bacillus cereus [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 Priestia megaterium [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Bacillus cereus YB-4 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a low-molecular-weight, Mn = 20000 Bacillus cereus YB-4 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Priestia megaterium NBRC15308 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PhaRCBm synthesizes (R)-3-hydroxybutanoate, P(3HB), with a relatively high molecular weight, Mn = 890000 Priestia megaterium NBRC15308 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
additional information the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview Bacillus cereus ?
-
 ?
additional information the PhaC from BAcillus cereus also shows a polyhydroxybutanoate hydrolyzing activity, time-dependent change in inverse of degree of polymerization during intracellular P(3HB) degradation at a culture temperature of 37°C, overview Bacillus cereus YB-4 ?
-
 ?

Synonyms

Synonyms Comment Organism
class IV PHA synthase
-
 Priestia megaterium
class IV PHA synthase
-
 Bacillus cereus
PhaRCBm
-
 Priestia megaterium
PhaRCYB4
-
 Bacillus cereus