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Literature summary for 2.3.1.304 extracted from
- Characterization and properties of G4X mutants of Ralstonia eutropha PHA synthase for poly(3-hydroxybutyrate) biosynthesis in Escherichia coli (2005), Macromol. Biosci., 5, 197-206.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and (His)6-tagged PhaCRe, expression in Escherichia coli. Wild-type enzyme expressed in Escherichia coli shows 35% of the enzyme from Ralstonia eutropha | Cupriavidus necator |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F396L | about 40% of the poly(3-hydroxybutyrate) content compared to wild-type | Cupriavidus necator |
| G4A | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 14% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4C | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 24% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4D | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4E | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4F | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 45% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4H | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4I | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type | Cupriavidus necator |
| G4K | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4L | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 2% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4M | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 24% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4N | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 57% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4P | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4Q | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 55% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type | Cupriavidus necator |
| G4R | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4S | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type | Cupriavidus necator |
| G4T | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type | Cupriavidus necator |
| G4V | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 12% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4W | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 13% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| G4Y | poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type | Cupriavidus necator |
| N208D | about 40% of the poly(3-hydroxybutyrate) content compared to wild-type | Cupriavidus necator |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
(R)-3-hydroxybutyryl-CoA | 25°C, pH 7.0, wild-type enzyme, based on one catalytic site in a dimeric enzyme | Cupriavidus necator |  |
| 0.32 | - |
(R)-3-hydroxybutyryl-CoA | 25°C, pH 7.0, mutant enzyme G4D, based on one catalytic site in a dimeric enzyme | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, (His)6-tagged wild-type and G4D mutant PhaCRe, SDS-PAGE | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n | - |
Cupriavidus necator | [(R)-3-hydroxybutanoate](n+1) + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, (His)6-tagged wild-type and G4D mutant PhaCRe, SDS-PAGE | Cupriavidus necator |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhaCRe | - |
Cupriavidus necator |
| type I polyhydroxyalkanoate synthase | - |
Cupriavidus necator |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
(R)-3-hydroxybutyryl-CoA | 25°C, pH 7.0, mutant enzyme G4D, based on one catalytic site in a dimeric enzyme | Cupriavidus necator | |
| 44 | - |
(R)-3-hydroxybutyryl-CoA | 25°C, pH 7.0, wild-type enzyme, based on one catalytic site in a dimeric enzyme | Cupriavidus necator | |
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