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Literature summary for 2.3.1.304 extracted from

  • Liebergesell, M.; Sonomoto, K.; Madkour, M.; Mayer, F.; Steinbuchel, A.
    Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules (1994), Eur. J. Biochem., 226, 71-80.
    View publication on PubMed

General Stability

General Stability Organism
loss of specific activity of the PHA synthase is observed during purification at 4°C Allochromatium vinosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
(R)-3-hydroxybutyryl-CoA
-
Allochromatium vinosum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Allochromatium vinosum 5737
-
poly(hydroxyalkanoic acid) granule surface Allochromatium vinosum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
390000
-
non-denaturing PAGE Allochromatium vinosum
400000
-
gel filtration Allochromatium vinosum

Organism

Organism UniProt Comment Textmining
Allochromatium vinosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Allochromatium vinosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
Allochromatium vinosum [(R)-3-hydroxybutanoate](n+1) + CoA
-
?

Synonyms

Synonyms Comment Organism
PHA synthase
-
Allochromatium vinosum
PhaEC
-
Allochromatium vinosum
poly(hydroxyalkanoic acid) synthase
-
Allochromatium vinosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 purified PHA synthase exhibits no significant loss of specific activity Allochromatium vinosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
in sodium phosphate as well as in Tris/HCl buffer Allochromatium vinosum

pH Range

pH Minimum pH Maximum Comment Organism
7 8.8 pH 7.0: about 40% of maximal activity, pH 8.8: about 65% of maximal activity, Tris/HCl buffer Allochromatium vinosum