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Literature summary for 2.3.1.304 extracted from

  • Normi, Y.M.; Hiraishi, T.; Taguchi, S.; Sudesh, K.; Najimudin, N.; Doi, Y.
    Site-directed saturation mutagenesis at residue F420 and recombination with another beneficial mutation of Ralstonia eutropha polyhydroxyalkanoate synthase (2005), Biotechnol. Lett., 27, 705-712.
    View publication on PubMed
Search for more literature for 2.3.1.304

Protein Variants

Protein Variants Comment Organism
F420A poly(3-hydroxybutyrate) content is about 20% of wild-type value Cupriavidus necator
F420C poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
f420D poly(3-hydroxybutyrate) content is about 20% of wild-type value Cupriavidus necator
F420E poly(3-hydroxybutyrate) content is about 10% of wild-type value Cupriavidus necator
F420G poly(3-hydroxybutyrate) content is about 20% of wild-type value Cupriavidus necator
F420H poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
F420I poly(3-hydroxybutyrate) content is about 30% of wild-type value Cupriavidus necator
F420K poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
F420L poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
F420M poly(3-hydroxybutyrate) content is about 40% of wild-type value Cupriavidus necator
F420N poly(3-hydroxybutyrate) content is about 45% of wild-type value Cupriavidus necator
F420Q poly(3-hydroxybutyrate) content is about 30% of wild-type value Cupriavidus necator
F420R poly(3-hydroxybutyrate) content is about 30% of wild-type value Cupriavidus necator
F420S F420S enzyme has a significant decrease in its lag phase compared to that of the wild-type enzyme. Poly(3-hydroxybutyrate) content is about 85% of wild-type value Cupriavidus necator
F420T poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
F420V poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
F420W poly(3-hydroxybutyrate) content is about 25% of wild-type value Cupriavidus necator
F420Y poly(3-hydroxybutyrate) content is about 35% of wild-type value Cupriavidus necator
G4D/F420S mutant shows a higher poly(3-hydroxybutyrate) content and in vivo concentration of PhaCRe enzyme than the F420S mutant, the molecular weight of the poly(3-hydroxybutyrate) polymer of the double mutant is similar to that of the F420S mutant Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Cupriavidus necator [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?

Synonyms

Synonyms Comment Organism
PhaCRe
-
 Cupriavidus necator