Cloned (Comment) | Organism |
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the wild-type and mutated PHA synthase genes from Aeromonas caviae are introduced into Arabidopsis thaliana together with the NADPH-dependentacetoacetyl-CoA reductase gene from Ralstonia eutropha. Expression of the highly active mutated PHA synthase genes, N149S and D171G, leads to an 8-10fold increase in PHA content in the T1 transgenic Arabidopsis, compared to plants harboring the wild-type PHA synthase gene. In homozygous T2 progenies, PHA content is further increased up to 6.1 mg/g cell dry weight. GC/MS analysis of the purified PHA from the transformants revealed that these PHAs are poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers consisting of 0.2-0.8 mol% 3-hydroxyvalerate | Aeromonas caviae |
Organism | UniProt | Comment | Textmining |
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Aeromonas caviae | - |
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