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Literature summary for 2.3.1.304 extracted from

  • Yuan, W.; Jia, Y.; Tian, J.; Snell, K.D.; Muh, U.; Sinskey, A.J.; Lambalot, R.H.; Walsh, C.T.; Stubbe, J.
    Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies (2001), Arch. Biochem. Biophys., 394, 87-98.
    View publication on PubMed
Search for more literature for 2.3.1.304

Cloned(Commentary)

Cloned (Comment) Organism
construction of (His)6-tagged Ralstonia eutropha PHA synthase gene, expression in Escherichia coli Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 (R)-3-hydroxybutyryl-CoA 30°C, first phase of reaction Allochromatium vinosum
0.18
-
 (R)-3-hydroxypentanoyl-CoA 30°C, first phase of reaction Allochromatium vinosum
0.32
-
 (R)-3-hydroxypentanoyl-CoA 30°C, second phase of reaction Allochromatium vinosum
0.45
-
 (R)-3-hydroxybutyryl-CoA 30°C, second phase of reaction Allochromatium vinosum

Organism

Organism UniProt Comment Textmining
Allochromatium vinosum
-
-
-
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Cupriavidus necator [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Allochromatium vinosum [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
(R)-3-hydroxypentanoyl-CoA + [(R)-3-hydroxypentanoate]n activity is 10% of the reaction with 3-hydroxybutyryl-CoA Cupriavidus necator [(R)-3-hydroxypentanoate](n+1)
-
 ?
(R)-3-hydroxypentanoyl-CoA + [(R)-3-hydroxypentanoate]n activity is 43% of the reaction with 3-hydroxybutyryl-CoA Allochromatium vinosum [(R)-3-hydroxypentanoate](n+1)
-
 ?
additional information less than 2% of the activity with 3-hydroxybutyryl-CoA: (R)-3-hydroxyhexanoyl-CoA, 3-hydroxypropionyl-CoA, (2R,3R)-2-methyl-3-hydroxylbutyryl-CoA, (R)-3-hydroxybutyryl (D)-pantetheine thioester, (R)-lactyl-CoA Allochromatium vinosum ?
-
 ?
additional information no or less than 1% of the activity with 3-hydroxybutyryl-CoA: (R)-3-hydroxyhexanoyl-CoA, 3-hydroxypropionyl-CoA, (S)-3-hydroxybutyryl-CoA, (2R,3R)-2-methyl-3-hydroxylbutyryl-CoA, (R)-3-hydroxybutyryl (D)-pantetheine thioester, 4-hydroxybutyryl-CoA, (R)-lactyl-CoA Cupriavidus necator ?
-
 ?

Synonyms

Synonyms Comment Organism
class I PHA synthase
-
 Cupriavidus necator
class I PHA synthase
-
 Allochromatium vinosum
class I PhaCRe
-
 Cupriavidus necator
PhaCAv
-
 Allochromatium vinosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.43
-
 (R)-3-hydroxypentanoyl-CoA 30°C, second phase of reaction Allochromatium vinosum
2 8 (R)-3-hydroxypentanoyl-CoA 30°C, first phase of reaction Allochromatium vinosum
18.67
-
 (R)-3-hydroxybutyryl-CoA 30°C, second phase of reaction Allochromatium vinosum
65.33
-
 (R)-3-hydroxybutyryl-CoA 30°C, first phase of reaction Allochromatium vinosum