Cloned (Comment) | Organism |
---|---|
gene cysE or MSMEG_5947, DNA and amino acid sequence determination and analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a MSMEG_5947 knockout mutant strain through homologous recombination, the mutant strain grows slowlier than the wild-type strain, and the lack of the CysE protein causes drastic morphological changes, overview | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26200 | - |
x * 26200, recombinant His-tagged enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Mycolicibacterium smegmatis | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Mycolicibacterium smegmatis ATCC 700084 | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R4T4 | gene cysE or MSMEG_5947 | - |
Mycolicibacterium smegmatis ATCC 700084 | A0R4T4 | gene cysE or MSMEG_5947 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Mycolicibacterium smegmatis | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Mycolicibacterium smegmatis ATCC 700084 | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26200, recombinant His-tagged enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
CysE | - |
Mycolicibacterium smegmatis |
serine acetyltransferase | - |
Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
malfunction | the MSMEG_5947 knockout mutant strain grows slower than the wild-type strain, and the lack of the CysE protein causes drastic morphological changes. Deletion of the serine acetyltransferase retards the growth of the organism, but serine acetyltransferase expression is not essential for the survival of the bacterium | Mycolicibacterium smegmatis |