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Literature summary for 2.3.1.30 extracted from
- Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene (2013), Int. J. Mol. Med., 31, 1229-1233.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cysE or Rv2335, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Mycobacterium tuberculosis | |
| 0.0264 | - |
L-serine | pH 7.5, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis |  |
| 0.0513 | - |
acetyl-CoA | pH 7.5, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+ has no effect on the enzyme activity | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24600 | - |
x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation | Mycobacterium tuberculosis |
| 30000 | - |
x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | Mycobacterium tuberculosis | - |
CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | Mycobacterium tuberculosis H37Rv | - |
CoA + O-acetyl-L-serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P95231 | gene Rv2335 or cysE | - |
| Mycobacterium tuberculosis H37Rv | P95231 | gene Rv2335 or cysE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.66 | - |
purified recombinant His-tagged enzyme, pH 7.5, 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | - |
Mycobacterium tuberculosis | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | - |
Mycobacterium tuberculosis H37Rv | CoA + O-acetyl-L-serine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, recombinant His-tagged enzyme, SDS-PAGE, x * 24600, about, sequence calculation | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CysE | - |
Mycobacterium tuberculosis |
| serine acetyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant enzyme | Mycobacterium tuberculosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | 50% of maximal activity at 20°C and 80°C | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant enzyme | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 50% of maximal activity at pH 5.0 and pH 10.0 | Mycobacterium tuberculosis |
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