Literature summary for 2.3.1.30 extracted from

Tavares, S.; Wirtz, M.; Beier, M.P.; Bogs, J.; Hell, R.; Amancio, S.
Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants (2015), Front. Plant Sci., 6, 74.

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Cloned(Commentary)

Cloned (Comment)	Organism
isozyme VvSERAT1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme	Vitis vinifera
isozyme VvSERAT2-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme	Vitis vinifera
isozyme VvSERAT2-2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme	Vitis vinifera
isozyme VvSERAT3-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme	Vitis vinifera

Inhibitors

Inhibitors	Comment	Organism	Structure
L-cysteine	feedback inhibition; feedback inhibition; feedback inhibition; feedback inhibition, isozyme VvSERAT2-1 is almost insensitive to cysteine inhibition	Vitis vinifera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	VvSERAT2-1, mainly	Vitis vinifera	9507	-
cytosol	-	Vitis vinifera	5829	-
cytosol	isozyme VvSERAT1-1	Vitis vinifera	5829	-
cytosol	isozyme VvSERAT3-1	Vitis vinifera	5829	-
mitochondrion	isozyme VvSERAT2-2, mainly	Vitis vinifera	5739	-
additional information	isozyme subcellular localization and expression analysis	Vitis vinifera	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
294600	-	x * 294600, sequence calculation	Vitis vinifera
323400	-	x * 323400, sequence calculation	Vitis vinifera
323800	-	x * 323800, sequence calculation	Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + L-serine	Vitis vinifera	-	CoA + O-acetyl-L-serine	-	?

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	-	cv. Touriga Nacional, isozyme VvSERAT1-1	-
Vitis vinifera	A0A0C5DAX6	isozyme VvSERAT2-2; cv. Touriga Nacional, isozyme VvSERAT2-2	-
Vitis vinifera	A0A0C5DES6	isozyme VvSERAT3-1; cv. Touriga Nacional, isozyme VvSERAT3-1	-
Vitis vinifera	B0LLV2	cv. Touriga Nacional, isozyme VvSERAT2-1	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
25	-	recombinant enzyme, isozyme VvSERAT2-2 in the cysteine synthase complex, pH and temperature not specified in the publication	Vitis vinifera

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-serine	-	Vitis vinifera	CoA + O-acetyl-L-serine	-	?

Subunits

Subunits	Comment	Organism
?	x * 294600, sequence calculation	Vitis vinifera
?	x * 323400, sequence calculation	Vitis vinifera
?	x * 323800, sequence calculation	Vitis vinifera

Synonyms

Synonyms	Comment	Organism
SERAT	-	Vitis vinifera
serine acetyltransferase	-	Vitis vinifera

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.16	-	isozyme VvSERAT2-2, pH and temperature not specified in the publication	Vitis vinifera	L-cysteine
1.9	-	isozyme VvSERAT2-1, pH and temperature not specified in the publication	Vitis vinifera	L-cysteine

Expression

Organism	Comment	Expression
Vitis vinifera	the isozyme expression of VvSERAT1-1 is not affected by sufate depletion	additional information
Vitis vinifera	the isozyme expression of VvSERAT2-2 is not affected by sufate depletion	additional information
Vitis vinifera	the isozyme expression of VvSERAT3-1 is not affected by sufate depletion	additional information
Vitis vinifera	isoform-specific induction of VvSERAT2-1 in leaves upon drought,whereas highlight- or temperature-stress hardly trigger VvSERAT2-1 transcription. Prolonged sulfate depletion, which is accompanied with decreased cysteine levels, causes 3.9-4.5fold increase of VvSERAT2-1 mRNA steady state levels at day five and seven, respectively	up

General Information

General Information	Comment	Organism
evolution	the enzyme is a member of the VvSERAT protein family, the identified four members of the VvSERAT protein family are assigned to three distinct groups upon their sequence similarities to Arabidopsis SERATs	Vitis vinifera
metabolism	serine acetyltransferase provides O-acetylserine for the biosynthesis of cysteine and thereby regulates the activity of O-acetylserine(thiol)lyase, EC 2.5.1.47, which catalyzes the last step of cysteine biosynthesis and reversibly interacts with serine acetyltransferase in the cysteine synthase complex	Vitis vinifera
metabolism	serine acetyltransferase provides O-acetylserine for the biosynthesis of cysteine and thereby regulates the activity of O-acetylserine(thiol)lyase, EC 2.5.1.47, which catalyzes the last step of cysteine biosynthesis and reversibly interacts with serine acetyltransferase in the cysteine synthase complex. Only isozyme VvSERAT2-1 lacks the canonical C-terminal tail of plant SERATs and does not form the cysteine synthase complex and is almost insensitive to cysteine inhibition	Vitis vinifera
additional information	the enzyme reversibly interacts with O-acetylserine(thiol)lyase in the cysteine synthase complex	Vitis vinifera

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Release 2023.1 (January 2023)