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Literature summary for 2.3.1.30 extracted from

  • Mino, K.; Hiraoka, K.; Imamura, K.; Sakiyama, T.; Eisaki, N.; Matsuyama, A.; Nakanishi, K.
    Characteristics of serine acetyltransferase from Escherichia coli deleting different lengths of amino acid residues from the C-terminus (2000), Biosci. Biotechnol. Biochem., 64, 1874-1880.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal deletion mutants, expression in Escherichia coli strain JM70 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants lacking 10 to 25 amino acid residues at the C-terminal end, deletions alter the sensitivity against L-cysteine inhibition and the interaction with O-acetylserine (thiol) lyase, overview, DELTAC30 mutant is inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Escherichia coli
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants from E. coli Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + L-serine = CoA + O-acetyl-L-serine binding motif for O-acetylserine (thiol) lyase is located within 10 amino acid residues at the C-terminal end Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Escherichia coli CoA + O-acetyl-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
SAT
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 SAT DELTAC10 mutant, lacking 10 amino acid residues at the C-terminal end is inactivated after 12 h Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli