Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli, construction of an expression vector which increases the expression to 17% of the soluble cell protein | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, 8-16% polyethylene glycol 1000, 10 mM Tris-HCl, pH 7.5, 2-4 weeks, room temperature, structure analysis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29261 | - |
4 * 29261, ultracentrifugation, gel filtration, crystallographic data | Escherichia coli |
125000 | - |
ultracentrifugation experiments | Escherichia coli |
160000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.6 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Escherichia coli | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Escherichia coli B / ATCC 11303 | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29261, ultracentrifugation, gel filtration, crystallographic data | Escherichia coli |