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Literature summary for 2.3.1.298 extracted from

  • Lim, X.Y.; Pickford, R.; Don, A.S.
    Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry (2016), Methods Mol. Biol., 1376, 11-22 .
    View publication on PubMed
Search for more literature for 2.3.1.298

Inhibitors

Inhibitors Comment Organism Structure
fumonisin B1
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens CERS3 transfers much longer chain fatty acids (C26, C28), forming highly hydrophobic ceramides that are a very important part of the water barrier function of skin ?
-
 ?
sphingosine + cerotoyl-CoA Homo sapiens
-
 N-cerotoylsphingosine + CoA
-
 ?
sphingosine + montanoyl-CoA Homo sapiens
-
 N-montanoylsphingosine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IU89
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CERS3 transfers much longer chain fatty acids (C26, C28), forming highly hydrophobic ceramides that are a very important part of the water barrier function of skin Homo sapiens ?
-
 ?
additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?
sphingosine + cerotoyl-CoA
-
 Homo sapiens N-cerotoylsphingosine + CoA
-
 ?
sphingosine + montanoyl-CoA
-
 Homo sapiens N-montanoylsphingosine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 3
-
 Homo sapiens
CerS3
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens