Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.297 extracted from

  • Couttas, T.A.; Lim, X.Y.; Don, A.S.
    A three-step assay for ceramide synthase activity using a fluorescent substrate and HPLC (2015), Lipids, 50, 101-109 .
    View publication on PubMed
Search for more literature for 2.3.1.297

Cloned(Commentary)

Cloned (Comment) Organism
gene CERS2, recombinant expression in HEK-293 cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrosphingosine + lignoceroyl-CoA Homo sapiens
-
 N-lignoceroyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + nervonoyl-CoA Homo sapiens
-
 N-nervonoyldihydrosphingosine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96G23
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
U-87MG cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrosphingosine + lignoceroyl-CoA
-
 Homo sapiens N-lignoceroyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + lignoceroyl-CoA low activity Homo sapiens N-lignoceroyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + nervonoyl-CoA
-
 Homo sapiens N-nervonoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + nervonoyl-CoA highly preferred substrate Homo sapiens N-nervonoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + palmitoyl-CoA low activity Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
dihydrosphingosine + stearoyl-CoA low activity Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
additional information CERS2 preferentially transfers very long chain fatty acids (C22:0-C26:0) to dihydrosphingosine. Improvement of a fluorescent assay, using HPLC using C16:0, C18:0 and C24:1 fatty acids and commercially available sphinganine-NBD, i.e. 7-nitro-2-1,3-benzoxadiazole-labelled dihydrosphingosine or (2S,3R)-2-amino-18((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)octadecane-1-3-diol, as substrates, very accurate and sensitive method for quantification of CERS activity, method evaluation Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 2
-
 Homo sapiens
CerS2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function very long chain ceramides synthesized by CERS2 are essential constituents of myelin. CERS2 demonstrates a preference for the monounsaturated C24:1 fatty acid substrate compared to the saturated C24:0 substrate, potentially explaining why myelin is enriched in ceramides containing the monounsaturated form of very long chain fatty acids Homo sapiens