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Literature summary for 2.3.1.297 extracted from

  • Kageyama-Yahara, N.; Riezman, H.
    Transmembrane topology of ceramide synthase in yeast (2006), Biochem. J., 398, 585-593 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.297

Cloned(Commentary)

Cloned (Comment) Organism
genes LAG1, LAC1 and LIP1, encodes the three subunits of the hetereotrimeric enzyme, sequence comparisons, recombinant expression of wild-type and point/insertion mutant FLAG- and/or c-Myc-tagged subunits in Saccharomyces cerevisiae strains, overview Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information topology study of Lag1p and Lac1p using insertion of glycosylation sites and fXa (factor Xa)-cleavage sites in the loops between the predicted transmembrane segments, hydropathy profiles of Lag1p and Lac1p and positions of fusion insertions, method, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane transmembrane topology of topology of the Lag1p and Lac1p subunits in yeast using insertion of glycosylation sites and factor Xa-cleavage sites in the loops between the predicted transmembrane segments, method, overview. The N- and C-termini of the proteins are in the cytoplasm and eight putative membrane-spanning domains are identified in Lag1p and Lac1p. The conserved Lag motif, potentially containing the active site, is most likely embedded in the membrane Saccharomyces cerevisiae 5789
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P28496 AND P38703 AND Q03579 encoding subunits Lac1p, Lag1p, and Lip1p
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Saccharomyces cerevisiae RH382 P28496 AND P38703 AND Q03579 encoding subunits Lac1p, Lag1p, and Lip1p
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Synonyms

Synonyms Comment Organism
ceramide synthase
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 Saccharomyces cerevisiae
Lac1p/Lag1p
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 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
additional information the conserved Lag motif, potentially containing the active site, is most likely embedded in the membrane. Histidine and aspartic acid residues in the Lag motif are essential for the function of Lag1p in vivo Saccharomyces cerevisiae
physiological function proteins Lag1p, Lac1p, and Lip1p are required for ceramide synthase activity in Saccharomyces cerevisiae Saccharomyces cerevisiae