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Literature summary for 2.3.1.297 extracted from

  • Mizutani, Y.; Kihara, A.; Igarashi, Y.
    Mammalian Lass6 and its related family members regulate synthesis of specific ceramides (2005), Biochem. J., 390, 263-271 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.297

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information Lass4 overproduction causing increases in both middle- and long-chain ceramides Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q924Z4
-
-
Mus musculus Q9D6J1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the N-glycosylation of Lass2 appears as three bands, deglycosylation of Lass2 with PNGase F (N-glycosidase F) Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney high expression level Mus musculus
-
liver high expression level Mus musculus
-
additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. Lass2 shows the highest expression in liver, but also high expression in kidney Mus musculus
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additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. Lass4 is strongly expressed in skin, with no apparent expression in muscle, testis, or thymus Mus musculus
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skin high expression level Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information both C22:0- and C24:0-CoAs are used effectively for Lass2- and Lass4-dependent ceramide synthesis, and C26:0-, C20:0-, and C18:0-CoAs also act as substrates, albeit weakly. Recombinant Lass2 produces longer ceramides such as C22:0 ceramides and C24:0 ceramides in transgenic HEK-293T cells Mus musculus ?
-
 ?
additional information both C22:0- and C24:0-CoAs are used effectively for Lass2- and Lass4-dependent ceramide synthesis, and C26:0-, C20:0-, and C18:0-CoAs also act as substrates, albeit weakly. Recombinant Lass4 produces longer ceramides such as C22:0 and C24:0 ceramides in transgenic HEK-293T cells Mus musculus ?
-
 ?
sphingosine + arachidoyl-CoA low activity Mus musculus N-arachidoylsphingosine + CoA
-
 ?
sphingosine + behenoyl-CoA
-
 Mus musculus N-behenoylsphingosine + CoA
-
 ?
sphingosine + hexacosanoyl-CoA low activity Mus musculus N-hexacosanoylsphingosine + CoA
-
 ?
sphingosine + lignoceroyl-CoA
-
 Mus musculus N-lignoceroylsphingosine + CoA
-
 ?
sphingosine + stearoyl-CoA low activity Mus musculus N-stearoylsphingosine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 2
-
 Mus musculus
ceramide synthase 4
-
 Mus musculus
CerS2
-
 Mus musculus
CerS4
-
 Mus musculus
Lass2
-
 Mus musculus
Lass4
-
 Mus musculus
trh1
-
 Mus musculus
trh3
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

General Information

General Information Comment Organism
evolution Lass proteins are known to contain a TLC [TRAM/Lag1p/CLN8 (ceroid-lipofuscinoses, neuronal 8)] homology domain with the Lag1 motif. Lass family members Lass2, Lass4 and Lass5, but not Lass1, also contain a HOX (homeobox) domain Mus musculus
malfunction Lass4 overproduction caused increases in both middle- and long-chain ceramides Mus musculus
additional information the N-terminus is essential for catalytic activity Mus musculus