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Literature summary for 2.3.1.294 extracted from

  • Machutta, C.A.; Bommineni, G.R.; Luckner, S.R.; Kapilashrami, K.; Ruzsicska, B.; Simmerling, C.; Kisker, C.; Tonge, P.J.
    Slow onset inhibition of bacterial beta-ketoacyl-acyl carrier protein synthases by thiolactomycin (2010), J. Biol. Chem., 285, 6161-6169 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.294

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli pLysS cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C171Q the mutant mimics the acyl-enzyme intermediate Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by thiolactomycin Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM] Mycobacterium tuberculosis
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM] Mycobacterium tuberculosis
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography and G-25 resin gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM]
-
 Mycobacterium tuberculosis an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?
an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM]
-
 Mycobacterium tuberculosis an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-AcpM synthase
-
 Mycobacterium tuberculosis
beta-ketoacyl-acyl carrier protein synthase
-
 Mycobacterium tuberculosis
KasB
-
 Mycobacterium tuberculosis