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Literature summary for 2.3.1.294 extracted from
- Substrate recognition by beta-ketoacyl-ACP synthases (2011), Biochemistry, 50, 10678-10686 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli pLysS cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R206G | the mutant shows a 10fold increase in kcat/Km when malonyl-CoA is varied at a fixed concentration of lauroyl-CoA (0.1 mM) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
malonyl-phosphopantetheine 14-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli |  |
| 0.0062 | - |
malonyl-phosphopantetheine 16-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.0237 | - |
malonyl-phosphopantetheine 8-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.0283 | - |
malonyl-CoA | mutant enzyme R206G, at pH 8.5 and 25°C | Escherichia coli | |
| 0.51 | - |
malonyl-CoA | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.59 | - |
malonyl-phosphopantetheine | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Escherichia coli | the enzyme synthesizes longer multi-unsaturated acyl chains averaging 54 carbons units | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | the enzyme synthesizes longer multi-unsaturated acyl chains averaging 54 carbons units | Escherichia coli | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| lauroyl-CoA + malonyl-CoA | 2% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli] | Escherichia coli | ? | - |
? | |
| lauroyl-CoA + malonyl-phosphopantetheine | 1% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli] | Escherichia coli | ? | - |
? | |
| lauroyl-CoA + malonyl-phosphopantetheine 14-mer | 108% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli] | Escherichia coli | ? | - |
? | |
| lauroyl-CoA + malonyl-phosphopantetheine 16-mer | 100% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli] | Escherichia coli | ? | - |
? | |
| lauroyl-CoA + malonyl-phosphopantetheine 8-mer | 22% activity compared to lauroyl-[acyl-carrier protein from Escherichia coli] plus malonyl-[acyl-carrier protein from Escherichia coli] | Escherichia coli | ? | - |
? | |
| lauroyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | 100% activity | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACP specific beta-ketoacyl synthase | - |
Escherichia coli |
| beta-ketoacyl-ACP synthase | - |
Escherichia coli |
| FabF | - |
Escherichia coli |
| KASII | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
malonyl-phosphopantetheine 8-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.021 | - |
malonyl-CoA | mutant enzyme R206G, at pH 8.5 and 25°C | Escherichia coli | |
| 0.022 | - |
malonyl-phosphopantetheine | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.022 | - |
malonyl-phosphopantetheine 16-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.023 | - |
malonyl-phosphopantetheine 14-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.042 | - |
malonyl-CoA | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme can catalyze both saturated and unsaturated fatty acid biosynthesis | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
malonyl-phosphopantetheine 16-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.037 | - |
malonyl-phosphopantetheine | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.083 | - |
malonyl-CoA | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 0.833 | - |
malonyl-CoA | mutant enzyme R206G, at pH 8.5 and 25°C | Escherichia coli | |
| 3.83 | - |
malonyl-phosphopantetheine 14-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
| 4.17 | - |
malonyl-phosphopantetheine 8-mer | wild type enzyme, at pH 8.5 and 25°C | Escherichia coli | |
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