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Literature summary for 2.3.1.294 extracted from

  • Sridharan, S.; Wang, L.; Brown, A.K.; Dover, L.G.; Kremer, L.; Besra, G.S.; Sacchettini, J.C.
    X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB) (2007), J. Mol. Biol., 366, 469-480.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.294

Application

Application Comment Organism
drug development the enzyme, along with the other enzyme involved in the FAS-II complex, is a potential target for development of anti-tubercular drugs Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
gene mtkasB, expression of His-tagged enzyme in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting drop vapour diffusion method, from 100 mM Caps, pH 10.5, 20% w/v PEG 8000, 200 mM NaCl, and 5 mM C16-CoA, with spermidine-HCl and Foscholine-9 detergent added, X-ray diffraction crystal structure determination and analysis at 2.4-3.0 A resolution, molecular replacement, structure modeling Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
thiolactomycin binding structure, modeling Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 recombinant enzyme, gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall ?
-
 ?
additional information Mycobacterium tuberculosis H37Rv the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 gene mtkasB, i.e. Rv2246
-
Mycobacterium tuberculosis H37Rv
-
 gene mtkasB, i.e. Rv2246
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration to near homogeneity Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
(Z)-hexadec-11-enoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] active site structure with the Cys-His-His active site triad and the acyl binding channel, reaction mechanism, modeling Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme catalyzes the Claisen-type condensation reaction Mycobacterium tuberculosis ?
-
 ?
additional information the beta-ketoacyl acyl carrier protein synthase II is a mycobacterial elongation condensing enzyme involved in FAS-II, FAS-II is considered to elongate medium chain length fatty acyl primers to provide long chain, C56, precursors of mycolic acids, mycolic acids are long chain alpha-alkyl branched, beta-hydroxy fatty acids that represent a characteristic component of the Mycobacterium tuberculosis cell wall Mycobacterium tuberculosis H37Rv ?
-
 ?
additional information the enzyme catalyzes the Claisen-type condensation reaction Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, crystal structure, mtKasB adopts a thiolase fold but contains unique structural features in the capping region that may be crucial to its preference for longer fatty acyl chains than its counterparts from other bacteria, residues 1-260 and 261-415 form topologically equivalent N and C-terminal betaalphabetaalphabetaalphabetabeta motifs, overview, structure comparisons Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
beta-ketoacyl acyl carrier protein synthase II
-
 Mycobacterium tuberculosis
KasB
-
 Mycobacterium tuberculosis