Literature summary for 2.3.1.293 extracted from

Luckner, S.; Machutta, C.; Tonge, P.; Kisker, C.
Crystal structures of Mycobacterium tuberculosis KasA show mode of action within cell wall biosynthesis and its inhibition by thiolactomycin (2009), Structure, 17, 1004-1013 .

Search for more literature for 2.3.1.293

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Mycobacterium smegmatis strain mc2155	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-enzyme and mutant C171Q bound to thiolactomycin, vapor diffusion method, using 10% (v/v) isopropanol, 0.2 M NaCl, 0.1 M HEPES pH 7.5 and 10 mM tris(2-carboxyethyl)phosphine hydrochloride (for apoenzyme), or 20% (w/v) polyethylene glycol 3350 and 0.2 M potassium formate (for mutant C171Q)	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
C171Q	acyl-enzyme mimic	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
thiolactomycin	-	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis	-	an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis H37Rv	-	an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WQD9	cf. EC 2.3.1.41	-
Mycobacterium tuberculosis H37Rv	P9WQD9	cf. EC 2.3.1.41	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography, MonoQ column chromatography and Superdex S200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis H37Rv	an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
beta-ketoacyl ACP synthase I	-	Mycobacterium tuberculosis
KasA	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)