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Literature summary for 2.3.1.293 extracted from

  • Machutta, C.A.; Bommineni, G.R.; Luckner, S.R.; Kapilashrami, K.; Ruzsicska, B.; Simmerling, C.; Kisker, C.; Tonge, P.J.
    Slow onset inhibition of bacterial beta-ketoacyl-acyl carrier protein synthases by thiolactomycin (2010), J. Biol. Chem., 285, 6161-6169 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.293

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Mycobacterium smegmatis strain mc2155 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C171Q the mutant mimics the acyl-enzyme intermediate Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
thiolactomycin slow onset inhibitor Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 palmitoyl-[acyl-carrier protein AcpM] at pH 8.5 and 25°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM] Mycobacterium tuberculosis
-
 an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM] Mycobacterium tuberculosis
-
 an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal ion affinity chromatography and G-25 resin gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein AcpM]
-
 Mycobacterium tuberculosis an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + palmitoyl-[acyl-carrier protein AcpM]
-
 Mycobacterium tuberculosis an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein AcpM]
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-AcpM synthase
-
 Mycobacterium tuberculosis
beta-ketoacyl-acyl carrier protein synthase
-
 Mycobacterium tuberculosis
KasA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.533
-
 palmitoyl-[acyl-carrier protein AcpM] at pH 8.5 and 25°C Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.252
-
 apoenzyme, at pH 8.5 and 25°C Mycobacterium tuberculosis thiolactomycin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
205
-
 palmitoyl-[acyl-carrier protein AcpM] at pH 8.5 and 25°C Mycobacterium tuberculosis